Western Blot (WB)
(Mouse Nek5 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek5 antibody detected the Nek5 protein (arrow).)
Rabbit anti-Mouse Nek5 Polyclonal Antibody | anti-Nek5 antibody
Nek5, CT (Nek5, Serine/threonine-protein kinase Nek5, Never in mitosis A-related kinase 5)
Gene Names
Nek5; BB049667; 6530411J22
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Nek5; Polyclonal Antibody; CT (Nek5; Serine/threonine-protein kinase Nek5; Never in mitosis A-related kinase 5); Anti -Nek5; anti-Nek5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Nek5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
Mouse Nek5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 524-552 amino acids from the C-terminal region of Mouse Nek5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Mouse Nek5 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek5 antibody detected the Nek5 protein (arrow).)
Western Blot (WB)
(Mouse Nek5 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek5 antibody detected the Nek5 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Nek5 Antibody (C-term) (AP14449b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Nek5 Antibody (C-term) (AP14449b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-Nek5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
71,790 Da
NCBI Official Full Name
Nek5 protein
NCBI Official Synonym Full Names
NIMA (never in mitosis gene a)-related expressed kinase 5
NCBI Official Symbol
Nek5
NCBI Official Synonym Symbols
BB049667; 6530411J22
NCBI Protein Information
serine/threonine-protein kinase Nek5; NIMA-related kinase 5; nimA-related protein kinase 5; never in mitosis A-related kinase 5
UniProt Protein Name
Serine/threonine-protein kinase Nek5
Protein Family
UniProt Gene Name
Nek5
UniProt Synonym Gene Names
NimA-related protein kinase 5
UniProt Entry Name
NEK5_MOUSE
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium
By similarity.
Sequence similarities: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.Contains 1 protein kinase domain.
Research Articles on Nek5
Similar Products
Product Notes
The Nek5 nek5 (Catalog #AAA649443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nek5, CT (Nek5, Serine/threonine-protein kinase Nek5, Never in mitosis A-related kinase 5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nek5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the Nek5 nek5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nek5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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