Cadherin, Placental (CDHP) Recombinant Protein | CDHP recombinant protein

Recombinant Cadherin, Placental (CDHP)

Cat. Num. AAA2010537

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cadherin; Placental (CDHP); Recombinant Cadherin; CDHP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-GDDGDHFTIT THPESNQGIL TTRKGLDFEA KNQHTLYVEV TNEAPFVLKL PTSTATIVVH VEDVNEAPVF VPPSKVVEVQ EGIPTGEPVC VYTAEDPDKE NQKISYRILR DPAGWLAMDP DSGQVTAVGT LDREDEQFVR NNIYEVMVLA MDNGSPPTTG TGTLLLTLID VNDHGPVPEP RQITICNQSP VRQVLNITDK DLSPHTSPFQ AQLTDDSDIY WTAEVNEEGD TVVLSLKKFL KQDTYDVHLS LSDHGNKEQL TVIRATVCDC HGHVETCPGP WKGGFILPVL
• Sequence Length: 829

• Applicable Applications for CDHP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gly371~Leu660 (Accession # P22223) with N-terminal His-Tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein
• Accurate Molecula Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 4.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 14589891
• NCBI GeneID: 1001
• NCBI Accession #: NP_001784.2
• NCBI GenBank Nucleotide #: NM_001793.4
• UniProt Accession #: P22223; Q05DI6; B2R6F4
• Molecular Weight: 33.4kDa
• NCBI Official Full Name: cadherin-3 preproprotein
• NCBI Official Synonym Full Names: cadherin 3, type 1, P-cadherin (placental)
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3; calcium-dependent adhesion protein, placental
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin
• UniProt Entry Name: CADH3_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: response to drug; positive regulation of insulin-like growth factor receptor signaling pathway; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; negative regulation of transforming growth factor-beta2 production; retinal homeostasis; hair cycle process; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; visual perception; keratinization; cell adhesion; homophilic cell adhesion

Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy