Rabbit anti-Human GDI1 Polyclonal Antibody | anti-GDI1 antibody

GDI1, CT (GDI1, GDIL, OPHN2, RABGDIA, XAP4, Rab GDP dissociation inhibitor alpha, Guanosine diphosphate dissociation inhibitor 1, Oligophrenin-2, Protein XAP-4) (FITC)

Cat. Num. AAA6301844

• Gene Names: GDI1; 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GDI1; Polyclonal Antibody; CT (GDI1; GDIL; OPHN2; RABGDIA; XAP4; Rab GDP dissociation inhibitor alpha; Guanosine diphosphate dissociation inhibitor 1; Oligophrenin-2; Protein XAP-4) (FITC); Protein XAP-4; anti-GDI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GDI1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GDI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-443 amino acids from the C-terminal region of human GDI1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GDI1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GDI1 antibody detected the GDI1 protein (arrow).)

Related Product Information for anti-GDI1 antibody

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.

Product Categories/Family for anti-GDI1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010). Massignan, T., et al. Mol. Cell Proteomics 9(4):611-622(2010). Chen, Y., et al. Biochem. J. 422(2):229-235(2009). Zhang, K.J., et al. Yi Chuan 30(5):590-594(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503971
• NCBI GeneID: 2664
• NCBI Accession #: NP_001484.1
• NCBI GenBank Nucleotide #: NM_001493.2
• UniProt Accession #: P31150; P50394; Q6FG50; Q7Z2G6; Q7Z2G9; Q7Z2H5; Q7Z2I6
• Molecular Weight: 50,583 Da
• NCBI Official Full Name: rab GDP dissociation inhibitor alpha
• NCBI Official Synonym Full Names: GDP dissociation inhibitor 1
• NCBI Official Symbol: GDI1
• NCBI Official Synonym Symbols: 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• NCBI Protein Information: rab GDP dissociation inhibitor alpha
• UniProt Protein Name: Rab GDP dissociation inhibitor alpha
• Protein Family: Guanosine nucleotide diphosphate dissociation inhibitor
• UniProt Gene Name: GDI1
• UniProt Synonym Gene Names: GDIL; OPHN2; RABGDIA; XAP4; Rab GDI alpha; GDI-1
• UniProt Entry Name: GDIA_HUMAN

Uniprot Description

GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: Golgi apparatus; neuron projection; protein complex; cytoplasm; midbody; cytosol

Molecular Function: protein binding; GDP-dissociation inhibitor activity; Rab GDP-dissociation inhibitor activity; Rab GTPase binding; GTPase activator activity

Biological Process: protein transport; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of axonogenesis; Rab protein signal transduction; response to calcium ion; signal transduction; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 41