Rabbit anti-Human CEBPE Polyclonal Antibody | anti-CEBPE antibody

CEBPE, CT (CEBPE, CCAAT/enhancer-binding protein epsilon) (Azide free) (HRP)

Cat. Num. AAA6284967

• Gene Names: CEBPE; CRP1; C/EBP-epsilon
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CEBPE; Polyclonal Antibody; CT (CEBPE; CCAAT/enhancer-binding protein epsilon) (Azide free) (HRP); CCAAT/enhancer-binding protein epsilon; anti-CEBPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CEBPE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CEBPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-232 amino acids from the C-terminal region of human CEBPE.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CEBPE (arrow) using rabbit polyclonal CEBPE Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CEBPE gene.)

Western Blot (WB)

(CEBPE Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CEBPE antibody detected the CEBPE protein (arrow).)

Western Blot (WB)

(CEBPE Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CEBPE antibody detected the CEBPE protein (arrow).)

Related Product Information for anti-CEBPE antibody

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.

Product Categories/Family for anti-CEBPE antibody

Antibodies; Binding Proteins

References

Prasad, R.B., et al. Blood (2009). In press: Papaemmanuil, E., et al. Nat. Genet. 41(9):1006-1010(2009). Bedi, R., et al. Blood 113(2):317-327(2009). Cloutier, A., et al. J. Immunol. 182(1):563-571(2009). Matsushita, H., et al. Oncogene 27(53):6749-6760(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 28872800
• NCBI GeneID: 1053
• NCBI Accession #: NP_001796.2
• NCBI GenBank Nucleotide #: NM_001805.3
• UniProt Accession #: Q15744; Q15745; Q8IYI2; Q99803
• Molecular Weight: 30,603 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein epsilon
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), epsilon
• NCBI Official Symbol: CEBPE
• NCBI Official Synonym Symbols: CRP1; C/EBP-epsilon
• NCBI Protein Information: CCAAT/enhancer-binding protein epsilon; c/EBP epsilon
• UniProt Protein Name: CCAAT/enhancer-binding protein epsilon
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPE
• UniProt Synonym Gene Names: C/EBP epsilon
• UniProt Entry Name: CEBPE_HUMAN

NCBI Description

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

C/EBP-epsilon: C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm

Molecular Function: protein homodimerization activity; protein heterodimerization activity

Biological Process: macrophage differentiation; transcription, DNA-dependent; cytokine biosynthetic process; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; defense response; phagocytosis

Disease: Specific Granule Deficiency