Rabbit anti-Human GCSF Polyclonal Antibody | anti-G-CSF antibody

Rabbit anti GCSF Polyclonal

Cat. Num. AAA551018

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Serum IgG fraction was purified by Protein G affinity chromatography.
• Synonyms: GCSF; Polyclonal Antibody; Rabbit anti GCSF Polyclonal; Rabbit anti GCSF Polyclonal purified; g-csf; gcsf; Granulocyte Colony Stimulating polyclonal; PAb; anti-G-CSF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum IgG fraction was purified by Protein G affinity chromatography.
• Form/Format: Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
• Sequence Length: 207

• Applicable Applications for anti-G-CSF antibody: Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
• Application Notes: ELISA: Use at a concentration range of 0.5-1ug/ml,The detection limit for human G-CSF is 0.00245 ng/well; Western blot:A suitable range of concentrations of this antibody for WB detection is 1-2 ug/ml.; IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
• Source Note: Polyclonal G-CSF antibody was produced from sera of rabbits immunized with recombinant human GCSF.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
• Preparation and Storage: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.; Shelf Life: >12 months

Related Product Information for anti-G-CSF antibody

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is produced also by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM- CSF.Comparison of the primary sequence of G-CSF with those of the two other colony stimulating factors, GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF show a sequence homology of approximately 70 % at the DNA level and of 72 % at the protein level. The G-CSF receptor,CD114, is expressed on all cells of the neutrophils and granulocytes lineage. It is expressed also in placenta cells, endothelial cells and various carcinoma cell lines.Human G-CSF is active in murine cells and vice versa. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. G-CSF synergises with some other cytokines, including GM-CSF and IL4. GM-CSF and G-CSF are required, for example, to develop neutrophilic colonies in vitro. The concerted action of G-CSF and Epo is required to support the growth of mixed colonies of the early erythroid progenitors. A combination of IL4 with G- CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines.

Product Categories/Family for anti-G-CSF antibody

Polyclonal

References

• plerixafor (amd3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different cd34+ cell populations based on global gene and microrna expression signatures .Blood, Sep 2009; 114: 2530 - 2541.
• the role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice .J. Neurosci., Sep 2009; 29: 11572 - 11581.
• nfi-a directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression .Blood, Aug 2009; 114: 1753 - 1763.
• silencing of microrna-155 in mice during acute inflammatory response leads to derepression of c/ebp beta and down-regulation of G-CSF .Nucleic Acids Res., Jul 2009; 10.1093/nar/gkp577.
• preoperative weekly cisplatin–epirubicin–paclitaxel with G-CSF support in triple-negative large operable breast cancer .Ann. Onc., Jul 2009; 20: 1185 - 1192.