Testing Data
Human G-CSF ELISA Kit | CSF3 elisa kit
Human G-CSF Quick ELISA Kit
O.D.|0.002|0.058|0.092|0.170|0.319|0.599|1.080|1.902
n|16|16|16|
Mean(pg/ml)|65|240|981|
Standard deviation|3.96|14.88|43.16|
CV(%)|6.1%|6.2%|4.4%|
n|24|24|24
Mean(pg/ml)|67|233|946
Standard deviation|5.15|18.17|61.49
CV(%)|7.7%|7.8%|6.5%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 65|55|63|58|60|2.52|4.2%
Sample 2: 240|210|242|227|229|11.45|5%
Sample 3: 981|965|892|988|956|43.02|4.5%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
specially designed to measure Human CSF3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CSF3. The detection antibody is a HRP linked goat polyclonal antibody specific for CSF3. The kit contains recombinant Human CSF3 with immunogen: Expression system for standard: E Coli; Immunogen sequence: T31-P204. The kit is analytically validated with ready to use reagents.
To measure Human CSF3, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CSF3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CSF3 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Uniprot Description
G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Cell cycle regulation; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: extracellular space
Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding
Biological Process: granulocyte differentiation; positive regulation of myeloid cell differentiation; positive regulation of protein binding; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter
Research Articles on CSF3
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Product Notes
The Human CSF3 csf3 (Catalog #AAA1753908) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753908 ELISA Kit recognizes Human CSF3. It is sometimes possible for the material contained within the vial of "G-CSF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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