Rabbit GBP2 Polyclonal Antibody | anti-GBP2 antibody

GBP2 antibody - N-terminal region

Cat. Num. AAA3211819

• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat; Tested Species Reactivity||Human
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: GBP2; Polyclonal Antibody; GBP2 antibody - N-terminal region; anti-GBP2 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat; Tested Species Reactivity||Human
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: HTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLS
• Sequence Length: 591

• Applicable Applications for anti-GBP2 antibody: Western Blot (WB)
• Homology: Cow: 86%; Dog: 79%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 86%
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GBP2
• Protein Size: 591 amino acids
• Protein Interactions: CNDP2; NANS; RPS6KA1; PEPD; MVD; GSR; GNS; GLA; G6PD; CASP7; CAPN1; ASS1; GSK3B; GADD45A; CSNK2B; BNIP3L; BID; ANXA7; MAPK8IP2; STK3; KLHL20; BTBD2; LSM2; PAFAH1B3; PPP1R8; MAGI1; TFG; SERPINB9; SEPHS1; FXR1; HSPE1; SAT1
• Preparation and Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-GBP2 antibodyFormalin Fixed Paraffin Embedded Tissue: Human ProstatePrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

Immunohistochemistry (IHC)

(Rabbit Anti-GBP2 antibodyFormalin Fixed Paraffin Embedded Tissue: Human SpleenPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

Western Blot (WB)

(WB Suggested Anti-GBP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate)

Related Product Information for anti-GBP2 antibody

This is a rabbit polyclonal antibody against GBP2. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP. Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP.

Product Categories/Family for anti-GBP2 antibody

Polyclonal; Lymphocyte Signaling; Chromatin & Nuclear Signaling; DNA/RNA/Protein Interactions

NCBI and Uniprot Product Information

• NCBI GI #: 38327558
• NCBI GeneID: 2634
• NCBI Accession #: NP_004111
• NCBI GenBank Nucleotide #: NM_004120
• UniProt Accession #: P32456
• Molecular Weight: 67kDa
• NCBI Official Full Name: guanylate-binding protein 2
• NCBI Official Synonym Full Names: guanylate binding protein 2
• NCBI Official Symbol: GBP2
• NCBI Protein Information: guanylate-binding protein 2
• UniProt Protein Name: Guanylate-binding protein 2
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP2
• UniProt Synonym Gene Names: GBP-2; HuGBP-2
• UniProt Entry Name: GBP2_HUMAN

NCBI Description

This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013]

Uniprot Description

GBP2: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Hydrolase; Vesicle

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: cytosol; Golgi membrane; perinuclear region of cytoplasm

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; immune response; metabolic process