Rabbit anti-Human GBP1 Polyclonal Antibody | anti-GBP1 antibody

Anti-GBP1 antibody

Cat. Num. AAA175232

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: GBP1; Polyclonal Antibody; Anti-GBP1 antibody; Interferon-induced guanylate-binding protein 1; guanylate binding protein 1; interferon-inducible; GBP 1 antibody; GBP-1 antibody; GBP1 antibody; GBP1_HUMAN antibody; GTP binding protein 1 antibody; GTP-binding protein 1 antibody; Guanine nucleotide binding protein 1 antibody; Guanine nucleotide-binding protein 1 antibody; Guanylate binding protein 1 antibody; Guanylate binding protein 1 interferon inducible 67kDa antibody; Guanylate binding protein 1 interferon inducible antibody; HuGBP 1 antibody; HuGBP-1 antibody; HuGBP1 antibody; Interferon induced guanylate binding protein 1 antibody; Interferon-induced guanylate-binding protein 1 antibody; OTTHUMP00000012352 antibody; anti-GBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 592

• Applicable Applications for anti-GBP1 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GBP1 (566-589aa QKESRIMKNEIQDLQTKMRRRKAC).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-GBP1 antibody, MBS175232, Western blottingAll lanes: Anti GBP1 (MBS175232) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD )

Related Product Information for anti-GBP1 antibody

Description: Rabbit IgG polyclonal antibody for Interferon-induced guanylate-binding protein 1(GBP1) detection. Tested with WB in Human.
Background: Interferons induce a large number of genes in their target cells, including those coding for guanylate-binding proteins(GBPs). GBPs, such as GBP1, are characterized by their ability to specifically bind guanine nucleotides(GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. The 593-amino acid GBP1 protein shares 77% and 88% identity with GBP2 and GBP3, respectively. All GBPs, including GBP1, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases. The GBP1 gene to the GBP gene cluster on chromosome 1p22.2. It is located telomeric to GBP2 and centromeric to GBP3. High expression of GBP1, GBP2, and GBP3 are in endothelial cells after stimulation with IFNG, TNF, or IL1B.

NCBI and Uniprot Product Information

• NCBI GI #: 311033383
• NCBI GeneID: 2633
• UniProt Accession #: P32455; Q5T8M1; D3DT26
• Molecular Weight: 67,931 Da
• NCBI Official Full Name: Interferon-induced guanylate-binding protein 1
• NCBI Official Synonym Full Names: guanylate binding protein 1, interferon-inducible
• NCBI Official Symbol: GBP1
• NCBI Protein Information: interferon-induced guanylate-binding protein 1; GBP-1; huGBP-1; GTP-binding protein 1; guanine nucleotide-binding protein 1; guanylate binding protein 1, interferon-inducible, 67kDa
• UniProt Protein Name: Interferon-induced guanylate-binding protein 1
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP1
• UniProt Synonym Gene Names: GBP-1; HuGBP-1
• UniProt Entry Name: GBP1_HUMAN

NCBI Description

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]

Uniprot Description

GBP1: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Vesicle; Cell adhesion; Hydrolase

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: Golgi membrane; extracellular region; cytosol

Molecular Function: GTPase activity; identical protein binding; GTP binding

Biological Process: metabolic process; cytokine and chemokine mediated signaling pathway; defense response to virus