Rabbit RANBP1 Polyclonal Antibody | anti-RANBP1 antibody

RANBP1 antibody - C-terminal region

Cat. Num. AAA3208041

• Gene Names: RANBP1; HTF9A
• Reactivity: Cow, Horse, Human, Yeast
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: RANBP1; Polyclonal Antibody; RANBP1 antibody - C-terminal region; anti-RANBP1 antibody

• Host: Rabbit
• Reactivity: Cow, Horse, Human, Yeast
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: KFEECRKEIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ
• Sequence Length: 201

• Applicable Applications for anti-RANBP1 antibody: Western Blot (WB)
• Homology: Cow: 79%; Horse: 79%; Human: 100%; Yeast: 90%
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RANBP1
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-RANBP1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human TestisPrimary antibody Concentration: 1:200Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

Western Blot (WB)

(WB Suggested Anti-RANBP1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: 721_B cell lysateRANBP1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

Related Product Information for anti-RANBP1 antibody

This is a rabbit polyclonal antibody against RANBP1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Categories/Family for anti-RANBP1 antibody

Polyclonal; Chromatin & Nuclear Signaling; Signal Transduction; Various; DNA/RNA/Protein Interactions

NCBI and Uniprot Product Information

• NCBI GI #: 4506407
• NCBI GeneID: 5902
• NCBI Accession #: NP_002873
• NCBI GenBank Nucleotide #: NM_002882
• UniProt Accession #: P43487
• Molecular Weight: 23kDa
• NCBI Official Full Name: ran-specific GTPase-activating protein isoform 2
• NCBI Official Synonym Full Names: RAN binding protein 1
• NCBI Official Symbol: RANBP1
• NCBI Official Synonym Symbols: HTF9A
• NCBI Protein Information: ran-specific GTPase-activating protein
• UniProt Protein Name: Ran-specific GTPase-activating protein
• Protein Family: Ran-specific GTPase-activating protein
• UniProt Gene Name: RANBP1
• UniProt Synonym Gene Names: RanBP1
• UniProt Entry Name: RANG_HUMAN

NCBI Description

This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

RANBP1: a Ran-binding protein that functions in nuclear trafficking, mitosis, chromosomal segregation, and ciliogenesis. Controls both nuclear import and export. Contains a Ran binding domain and a C-terminal nuclear export signal. Following export from the nucleus, forms a complex with RanGTP and XPO1/cargo, leading to dissociation of cargo from XPO1. Stimulates RanGAP1 association with RanGTP, facilitating RanGTP hydrolysis to RanGDP. Stabilizes and regulates the formation of a RanGDP-importin/NLS receptor-RanBP1 complex during nuclear import. Controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and DLG7, to mitotic microtubles and regulates chromosome segregation. Knock down or overexpression experiments affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia.Belongs to the RANBP1 family.

Protein type: G protein regulator, misc.; Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytoplasm; nuclear envelope; nucleus

Molecular Function: GDP-dissociation inhibitor activity; Ran GTPase binding; GTPase activator activity

Biological Process: intracellular transport; viral reproduction; regulation of catalytic activity; signal transduction; positive regulation of GTPase activity