Rabbit anti-Human BAALC Polyclonal Antibody | anti-BAALC antibody

BAALC, NT (Brain And Acute Leukemia Cytoplasmic Protein) (MaxLight 750)

Cat. Num. AAA6464627

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BAALC; Polyclonal Antibody; NT (Brain And Acute Leukemia Cytoplasmic Protein) (MaxLight 750); anti-BAALC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BAALC.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-BAALC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human BAALC (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BAALC antibody (N-term) in 293 cell line lysates (35ug/lane). BAALC (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with BAALC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using BAALC Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-BAALC antibody

Antibodies; Neuroscience

References

1. Kuila, N., Leuk. Res. 33(4), 594-596 (2009). 2. Satoskar, A.A., Gene Expr. Patterns 5(4), 463-473 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 13376200
• NCBI GeneID: 79870
• NCBI Accession #: NP_079088
• NCBI GenBank Nucleotide #: NM_024812.2
• UniProt Accession #: Q8WXS3; Q8WTP6; Q8WXS0; Q8WXS1; Q8WXS2; Q9HA93
• Molecular Weight: 17.7 kDa (165aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: brain and acute leukemia cytoplasmic protein isoform 1
• NCBI Official Synonym Full Names: BAALC, MAP3K1 and KLF4 binding
• NCBI Official Symbol: BAALC
• NCBI Protein Information: brain and acute leukemia cytoplasmic protein
• UniProt Protein Name: Brain and acute leukemia cytoplasmic protein
• Protein Family: Brain and acute leukemia cytoplasmic protein
• UniProt Gene Name: BAALC
• UniProt Entry Name: BAALC_HUMAN

NCBI Description

This gene was identified by gene expression studies in patients with acute myeloid leukemia (AML). The gene is conserved among mammals and is not found in lower organisms. Tissues that express this gene develop from the neuroectoderm. Multiple alternatively spliced transcript variants that encode different proteins have been described for this gene; however, some of the transcript variants are found only in AML cell lines. [provided by RefSeq, Jul 2008]

Uniprot Description

BAALC: May play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: membrane; cytoplasm