Western Blot (WB)
(GBA3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GBA3 antibody detected the GBA3 protein (arrow).)
Rabbit anti-Human GBA3 Polyclonal Antibody | anti-GBA3 antibody
GBA3 Antibody (C-term)
Western Blot (WB)
(GBA3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GBA3 antibody detected the GBA3 protein (arrow).)
Western Blot (WB)
(GBA3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GBA3 antibody detected the GBA3 protein (arrow).)
Immunohistochemistry (IHC)
(GBA3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GBA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GBA3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GBA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
predominantly liver enzyme that efficiently hydrolyzes
beta-D-glucoside and beta-D-galactoside, but not any known
physiologic beta-glycoside, suggesting that it may be involved in
detoxification of plant glycosides (de Graaf et al., 2001 [PubMed
11389701]). GBA3 also has significant neutral glycosylceramidase
activity (EC 3.2.1.62), suggesting that it may be involved in a
nonlysosomal catabolic pathway of glucosylceramide metabolism
(Hayashi et al., 2007 [PubMed 17595169]).
Noguchi, J., et al. Biochem. Biophys. Res. Commun. 374(3):549-552(2008)
Hayashi, Y., et al. J. Biol. Chem. 282(42):30889-30900(2007)
Tribolo, S., et al. J. Mol. Biol. 370(5):964-975(2007)
Beutler, E., et al. J. Lab. Clin. Med. 144(2):65-68(2004)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Uniprot Description
GBA3: Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta- glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Other Amino Acids Metabolism - cyanoamino acid; Carbohydrate Metabolism - starch and sucrose; Hydrolase; EC 3.2.1.21
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: cytosol
Molecular Function: beta-glucosidase activity; beta-galactosidase activity; glycosylceramidase activity
Biological Process: sphingolipid metabolic process; glycoside catabolic process; carbohydrate metabolic process; glycosylceramide catabolic process; glycosphingolipid metabolic process
Research Articles on GBA3
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Product Notes
The GBA3 gba3 (Catalog #AAA9213011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 296-325. The GBA3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GBA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GBA3 gba3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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