Rabbit anti-Human TTLL13 Polyclonal Antibody | anti-TTLL13 antibody

TTLL13, ID (TTLL13, Tubulin polyglutamylase TTLL13, Tubulin-tyrosine ligase-like protein 13) (Azide free) (HRP)

Cat. Num. AAA6360039

• Gene Names: TTLL13P; TTLL13
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TTLL13; Polyclonal Antibody; ID (TTLL13; Tubulin polyglutamylase TTLL13; Tubulin-tyrosine ligase-like protein 13) (Azide free) (HRP); Tubulin--tyrosine ligase-like protein 13; anti-TTLL13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TTLL13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TTLL13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 468-498 amino acids from the Central region of human TTLL13.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TTLL13 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TTLL13 antibody detected the TTLL13 protein (arrow).)

Flow Cytometry (FC/FACS)

(TTLL13 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TTLL13 antibody

Polyglutamylase which preferentially modifies alpha-tubulin. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step (By similarity).

Product Categories/Family for anti-TTLL13 antibody

Antibodies; Tubulin

References

Janke, C., et al. Science 308(5729):1758-1762(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 75677580
• NCBI GeneID: 440307
• NCBI Accession #: NP_001025135.2
• NCBI GenBank Nucleotide #: NM_001029964.2
• UniProt Accession #: A6NNM8
• Molecular Weight: 93645
• NCBI Official Synonym Full Names: tubulin tyrosine ligase like 13, pseudogene
• NCBI Official Symbol: TTLL13P
• NCBI Official Synonym Symbols: TTLL13
• UniProt Protein Name: Tubulin polyglutamylase TTLL13
• Protein Family: Tubulin polyglutamylase
• UniProt Gene Name: TTLL13
• UniProt Entry Name: TTL13_HUMAN