Mouse anti-Human CGGBP1 Monoclonal Antibody | anti-CGGBP1 antibody

Mouse Monoclonal [clone 1D11] (IgG1,k) to Human CGGBP1

Cat. Num. AAA246143

• Gene Names: CGGBP1; CGGBP; p20-CGGBP
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CGGBP1; Monoclonal Antibody; Mouse Monoclonal [clone 1D11] (IgG1; k) to Human CGGBP1; Anti-CGGBP1 Antibody (clone 1D11) IHC-plus; 20 kDa CGG-binding protein; CGGBP; p20-CGGBP; p20-CGGBP DNA-binding protein; CGG-binding protein 1; p20-CGG binding protein; Human CGGBP1; anti-CGGBP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1D11
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Sequence Length: 167

• Applicable Applications for anti-CGGBP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control K-562. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: CGGBP1 (NP_003654, 58 a.a. ~ 168 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: CGGBP1 antibody was raised against cGGBP1 (NP_003654, 58 a.a. ~ 168 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CGGBP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CGGBP1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(CGGBP1 monoclonal antibody clone 1D11 Western blot of CGGBP1 expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CGGBP1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 56550053
• NCBI GeneID: 8545
• NCBI Accession #: NP_003654.3
• NCBI GenBank Nucleotide #: NM_003663.3
• UniProt Accession #: Q9UFW8; O15183; D3DU38
• Molecular Weight: 18,820 Da
• NCBI Official Full Name: CGG triplet repeat-binding protein 1
• NCBI Official Synonym Full Names: CGG triplet repeat binding protein 1
• NCBI Official Symbol: CGGBP1
• NCBI Official Synonym Symbols: CGGBP; p20-CGGBP
• NCBI Protein Information: CGG triplet repeat-binding protein 1; CGG-binding protein 1; p20-CGG binding protein; 20 kDa CGG-binding protein; p20-CGGBP DNA-binding protein
• UniProt Protein Name: CGG triplet repeat-binding protein 1
• Protein Family: CGG triplet repeat-binding protein
• UniProt Gene Name: CGGBP1
• UniProt Synonym Gene Names: CGGBP; CGG-binding protein 1
• UniProt Entry Name: CGBP1_HUMAN

NCBI Description

CGGBP1 influences expression of the FMR1 gene (MIM 309550), which is associated with the fragile X mental retardation syndrome (MIM 300624), by specifically interacting with the 5-prime (CGG)n-3-prime repeat in its 5-prime UTR.[supplied by OMIM, Mar 2008]

Uniprot Description

CGGBP1: Binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. May play a role in regulating FMR1 promoter.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 3p11.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: double-stranded DNA binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter