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Western Blot (WB) (Western Blot: Sample: Recombinant GRPR, Mouse.)

Rabbit anti-Mouse Gastrin Releasing Peptide Receptor Polyclonal Antibody | anti-GRPR antibody

Gastrin Releasing Peptide Receptor (GRPR) Polyclonal Antibody

Gene Names
Grpr; GRP-R
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Gastrin Releasing Peptide Receptor; Polyclonal Antibody; Gastrin Releasing Peptide Receptor (GRPR) Polyclonal Antibody; BB2; GRP-preferring bombesin receptor; anti-GRPR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GRPR. It has been selected for its ability to recognize GRPR in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
384
Applicable Applications for anti-GRPR antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
GRPR (Met1~Leu116)
Quality Control
Content: The quality control contains recombinant GRPR (Met1~Leu116) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066267
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant GRPR, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant GRPR, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,215 Da
NCBI Official Full Name
gastrin-releasing peptide receptor
NCBI Official Synonym Full Names
gastrin releasing peptide receptor
NCBI Official Symbol
Grpr
NCBI Official Synonym Symbols
GRP-R
NCBI Protein Information
gastrin-releasing peptide receptor
UniProt Protein Name
Gastrin-releasing peptide receptor
UniProt Gene Name
Grpr
UniProt Synonym Gene Names
GRP-R

NCBI Description

This gene encodes a multipass membrane protein that functions as a receptor for gastrin releasing peptide. Signalling through this receptor is important in a variety of physiological processes, including responses to itching, satiety, and fear. [provided by RefSeq, May 2015]

Uniprot Description

GRPR: Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: X F4|X 75.63 cM

Cellular Component: integral component of membrane; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: bombesin receptor activity; G-protein coupled receptor activity; neuropeptide binding; neuropeptide receptor activity; peptide receptor activity, G-protein coupled; protein binding; signal transducer activity

Biological Process: G-protein coupled receptor signaling pathway; learning and/or memory; phospholipase C-activating G-protein coupled receptor signaling pathway; regulation of cell proliferation; response to external biotic stimulus; signal transduction; social behavior

Research Articles on GRPR

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Product Notes

The GRPR grpr (Catalog #AAA2032193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gastrin Releasing Peptide Receptor (GRPR) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Gastrin Releasing Peptide Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GRPR grpr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gastrin Releasing Peptide Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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