Chicken GAP43 Polyclonal Antibody | anti-GAP43 antibody

GAP43 Antibody

Cat. Num. AAA415549

• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: GAP43; Polyclonal Antibody; GAP43 Antibody; anti-GAP43 antibody

• Host: Chicken
• Clonality: Polyclonal
• Specificity: Rat and Mouse.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-GAP43 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: On western blots look for a major band at 43 kDa, depending on the species.; 1:250-500 for immunofluorescence staining and 1:500-2500 for western blotting.
• Immunogen: GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and which run anomolously on SDS-PAGE. Other examples are microtubule associated proteins MAP2 and tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

References

1. Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J Cell Biol. 89:86-95 (1981).