Mouse anti-Rat, mouse GAP43 Monoclonal Antibody | anti-GAP43 antibody

GAP43 Antibody

Cat. Num. AAA415230

• Gene Names: gap43; b-50; pp46
• Reactivity: Rat, mouse
• Applications: Immunofluorescence, Western Blot, Immunocytochemistry
• Purity: Affinity purified
• Synonyms: GAP43; Monoclonal Antibody; GAP43 Antibody; anti-GAP43 antibody

• Host: Mouse
• Reactivity: Rat, mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 210

• Applicable Applications for anti-GAP43 antibody: Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower. Avoid repeated freezing and thawing, store at 4 degree C or -20 degree C.
• Antibody Type: primary antibody
• Immunogen: GAP43 is very abundant nervous system protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomolously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2 and tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications. The HGNC name for this protein is GAP43.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

Testing Data #2

Related Product Information for anti-GAP43 antibody

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

References

1. Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J. Cell Biol. 89:86-95 (1981).2. Wiederkehr A, Staple J, Caroni P. The Motility-Associated Proteins GAP-43, MARCKS, and CAP-23 Share Unique Targeting and Surface Activity-Inducing Properties. Exp. Cell Res. 236:103-116 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 118404060
• NCBI GeneID: 779658
• NCBI Accession #: NP_001072212.1
• NCBI GenBank Nucleotide #: NM_001078744.1
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: gap43
• NCBI Official Synonym Symbols: b-50; pp46
• NCBI Protein Information: growth associated protein 43; neuromodulin
• Protein Family: Neuromodulin