Western Blot (WB) #2
Rabbit GAP43 Polyclonal Antibody | anti-GAP43 antibody
GAP43 Antibody
Gene Names
gap43; b-50; pp46
Applications
Immunofluorescence, Western Blot
Purity
Affinity purified
Synonyms
GAP43; Polyclonal Antibody; GAP43 Antibody; anti-GAP43 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
210
Applicable Applications for anti-GAP43 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
The affinity purified antibody can be diluted to 1:1,000-5,000 for immunofluorescence staining and 1:10,000-50,000 for western blotting.
Immunogen
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and which run anomolously on SDS-PAGE. Other examples are microtubule associated proteins MAP2 and tau and the various neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Storage Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C/1 year
Western Blot (WB) #2
Western Blot (WB) #2
References
1. Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J. Cell Biol. 89:86-95 (1981).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
growth associated protein 43
NCBI Official Synonym Full Names
growth associated protein 43
NCBI Official Symbol
gap43
NCBI Official Synonym Symbols
b-50; pp46
NCBI Protein Information
growth associated protein 43; neuromodulin
Protein Family
Similar Products
Product Notes
The GAP43 (Catalog #AAA415970) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GAP43 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). The affinity purified antibody can be diluted to 1:1,000-5,000 for immunofluorescence staining and 1:10,000-50,000 for western blotting. Researchers should empirically determine the suitability of the GAP43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAP43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
