Testing Data
(G8990-10 staining of cultured neurons.)
Rabbit Growth Associated Protein 43 Polyclonal Antibody | anti-GAP-43 antibody
Growth Associated Protein 43 (GAP-43) (MaxLight 490)
Gene Names
Gap43; Basp2
Reactivity
Rat, Mouse, Human, Bovine, Chicken
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
Growth Associated Protein 43; Polyclonal Antibody; Growth Associated Protein 43 (GAP-43) (MaxLight 490); anti-GAP-43 antibody
Host
Rabbit
Reactivity
Rat, Mouse, Human, Bovine, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Growth Associated Protein-43 (GAP-43). Species Crossreactivity: Reacts to a high degree with rat and mouse. Reacts to a lesser degree with human, bovine and chicken.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-GAP-43 antibody
FLISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
FLISA: >= 1:2000
ICC: >= 1:1000 on hippocampal neruons in culture and PC12 cells stimulated with NGF.
IHC: >= 1:1000 on 4% paraformaldehyde/PBS fixed or 3% paraformaldehyde/01% gluteraldehyde/PBS fixed tissue sections.
WB: >= 1:2000
Applications are based on unconjugated antibody.
ICC: >= 1:1000 on hippocampal neruons in culture and PC12 cells stimulated with NGF.
IHC: >= 1:1000 on 4% paraformaldehyde/PBS fixed or 3% paraformaldehyde/01% gluteraldehyde/PBS fixed tissue sections.
WB: >= 1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to rat GAP-43 (complete sequence).
Conjugate
MaxLight490
Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation; cultured neurons
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(G8990-10 staining of cultured neurons.)
Testing Data
(G8990-10 staining of cultured neurons.)
Product Categories/Family for anti-GAP-43 antibody
References
1. Song, H., et al., Nature Neuroscience 5: 438-445 (2002). 2. Bareyre, F., et al., J. Neuroscience 22: 7097-7110 (2002). 3. Iwata, N., et al., J. Neuroscience 24: 991-998 (2004). 4. Frappe, I., et al., J. Neuroscience Research 75: 667-677 (2004). 5. Boyd, J.G., et al., PNAS 101: 2162-2166 (2004). 6. Kolterud, A., et al., Development 131: 5319-5326 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,603 Da
NCBI Official Full Name
neuromodulin
NCBI Official Synonym Full Names
growth associated protein 43
NCBI Official Symbol
Gap43
NCBI Official Synonym Symbols
Basp2
NCBI Protein Information
neuromodulin; axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; growth accentuating protein 43; growth-associated protein 43; protein F1
UniProt Protein Name
Neuromodulin
UniProt Gene Name
Gap43
UniProt Entry Name
NEUM_RAT
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Product Notes
The GAP-43 gap43 (Catalog #AAA6479239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Growth Associated Protein 43 (GAP-43) (MaxLight 490) reacts with Rat, Mouse, Human, Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Associated Protein 43 can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). FLISA: >= 1:2000 ICC: >= 1:1000 on hippocampal neruons in culture and PC12 cells stimulated with NGF. IHC: >= 1:1000 on 4% paraformaldehyde/PBS fixed or 3% paraformaldehyde/01% gluteraldehyde/PBS fixed tissue sections. WB: >= 1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GAP-43 gap43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Associated Protein 43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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