Rabbit anti-Human, Mouse Galpha t2 Polyclonal Antibody | anti-Galphat2 antibody

Galpha t2 (V34) Polyclonal Antibody

Cat. Num. AAA3004406

• Gene Names: GNAT2; ACHM4; GNATC
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Galpha t2; Polyclonal Antibody; Galpha t2 (V34) Polyclonal Antibody; GGH; CONJUGASE; EC 3.4.19.9; GH; Folylpolygammaglutamyl Hydrolase; Gamma-Glu-X carboxypeptidase; gamma-glutamyl hydrolase; hGH; anti-Galphat2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Galpha t2 (V34) polyclonal antibody detects endogenous levels of Galpha t2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 354

• Applicable Applications for anti-Galphat2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Galpha t2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Galpha t2 (V34) pAb in paraffin-embedded human testis tissue.)

Related Product Information for anti-Galphat2 antibody

In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13. The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust. In the well characterized visual system, photorhodopsin catalyzes the exchange of guanine nucleotides bound to the visual transducin Galpha subunits (Galpha t1 in rod cells and Galpha t2 in cone cells).

NCBI and Uniprot Product Information

• NCBI GI #: 20330805
• NCBI GeneID: 2780
• NCBI Accession #: NP_005263.1
• NCBI GenBank Nucleotide #: NP_005263.1
• UniProt Accession #: P19087
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-2
• NCBI Official Synonym Full Names: G protein subunit alpha transducin 2
• NCBI Official Symbol: GNAT2
• NCBI Official Synonym Symbols: ACHM4; GNATC
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-2
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-2
• UniProt Gene Name: GNAT2
• UniProt Synonym Gene Names: GNATC
• UniProt Entry Name: GNAT2_HUMAN

NCBI Description

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones. [provided by RefSeq, Jul 2008]

Uniprot Description

G-alpha t2: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4). Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; plasma membrane; heterotrimeric G-protein complex

Molecular Function: GTPase activity; GTP binding; G-protein-coupled receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; G-protein coupled photoreceptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; visual perception; retinal cone cell development; metabolic process; response to light intensity; phototransduction; detection of light stimulus involved in visual perception; detection of chemical stimulus involved in sensory perception of bitter taste

Disease: Achromatopsia 4