Mouse anti-Human ARRB2 / Beta Arrestin 2 Monoclonal Antibody | anti-ARRB2 antibody

Mouse Monoclonal [clone 3G1] (IgG2a,k) to Human ARRB2 / Beta Arrestin 2

Cat. Num. AAA246362

• Gene Names: ARRB2; ARB2; ARR2; BARR2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ARRB2 / Beta Arrestin 2; Monoclonal Antibody; Mouse Monoclonal [clone 3G1] (IgG2a; k) to Human ARRB2 / Beta Arrestin 2; Anti-ARRB2 / Beta Arrestin 2 Antibody (clone 3G1) IHC-plus; ARRB2; Arrestin 3; Arrestin beta-2; ARB2; ARR2; BARR2; Arrestin; beta 2; Beta-arrestin-2; Human ARRB2; Beta Arrestin 2; anti-ARRB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G1
• Specificity: Human Beta-Arrestin 2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 409

• Applicable Applications for anti-ARRB2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: ARRB2 (AAH07427, 300 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ARRB2 / Beta Arrestin 2 antibody was raised against aRRB2 (AAH07427, 300 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Beta-Arrestin 2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Beta-Arrestin 2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ARRB2 on HeLa cell. [antibody concentration 10 ug/ml].)

Western Blot (WB)

(Western blot of ARRB2 expression in transfected 293T cell line. Lane 1: ARRB2 transfected lysate (46 KDa). Lane 2: Non-transfected lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ARRB2 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4757780
• NCBI GeneID: 409
• NCBI Accession #: NP_004304.1
• NCBI GenBank Nucleotide #: NM_004313.3
• UniProt Accession #: P32121; Q0Z8D3; Q2PP19; Q6ICT3; Q8N7Y2; Q9UEQ6; B4DLW0; B5B0C0; B7WPL3; D3DTK2; H0Y688
• Molecular Weight: 44,392 Da
• NCBI Official Full Name: beta-arrestin-2 isoform 1
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: ARRB2
• NCBI Official Synonym Symbols: ARB2; ARR2; BARR2
• NCBI Protein Information: beta-arrestin-2; arrestin 3; arrestin beta-2
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB2
• UniProt Synonym Gene Names: ARB2; ARR2
• UniProt Entry Name: ARRB2_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: postsynaptic membrane; endocytic vesicle; basolateral plasma membrane; cytoplasm; postsynaptic density; dendritic spine; plasma membrane; coated pit; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: angiotensin receptor binding; protein kinase B binding; protein domain specific binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; protein complex scaffold; type 1 angiotensin receptor binding; D1 dopamine receptor binding; receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; adult walking behavior; protein transport; receptor internalization; transforming growth factor beta receptor signaling pathway; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; Notch signaling pathway; negative regulation of interleukin-12 production; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; blood coagulation; positive regulation of calcium ion transport