Immunoprecipitation (IP)
(MSL2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-MSL2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MSL2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
Rabbit anti-Human MSL2L1/MSL2 Polyclonal Antibody | anti-MSL2 antibody
Anti-MSL2L1/MSL2 Antibody, Rabbit Polyclonal
Gene Names
MSL2; MSL-2; MSL2L1; RNF184
Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
MSL2L1/MSL2; Polyclonal Antibody; Anti-MSL2L1/MSL2 Antibody; Rabbit Polyclonal; MSL2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-MSL-2 Antibody; Anti-MSL2L1 Antibody; Anti-RNF184 Antibody; anti-MSL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MSL2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MSL2 antibody
Immunoprecipitation (IP)
Application Notes
IP: 1-4uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human MSL2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MSL2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(MSL2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-MSL2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MSL2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
Immunoprecipitation (IP)
(MSL2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-MSL2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MSL2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
Related Product Information for anti-MSL2 antibody
MSL2 was associated with those genes responding to the virus. Mechanistically, MSL2 could maintain HBV cccDNA stability through degradation of APOBEC3B by ubiquitylation in hepatoma cells. MSL2 is an E3 ligase that ubiquitylates itself and the other associated components when their stoichiometry is unbalanced, uncovering proteasome-dependent degradation as an additional layer of homeostatic control of MSL levels. MSL2, together with MSL1, has robust histone ubiquitylation activity that mainly targets nucleosomal H2B on lysine 34 (H2B K34ub), a site within a conserved basic patch on H2B tail.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,541 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MSL2 isoform 2
NCBI Official Synonym Full Names
male-specific lethal 2 homolog (Drosophila)
NCBI Official Symbol
MSL2
NCBI Official Synonym Symbols
MSL-2; MSL2L1; RNF184
NCBI Protein Information
E3 ubiquitin-protein ligase MSL2; MSL2-like 1; ring finger protein 184; male-specific lethal 2-like 1; male-specific lethal-2 homolog 1
UniProt Protein Name
E3 ubiquitin-protein ligase MSL2
Protein Family
UniProt Gene Name
MSL2
UniProt Synonym Gene Names
KIAA1585; MSL2L1; RNF184; MSL2-like 1; MSL-2
UniProt Entry Name
MSL2_HUMAN
Similar Products
Product Notes
The MSL2 msl2 (Catalog #AAA8101494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MSL2L1/MSL2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MSL2L1/MSL2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the MSL2 msl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSL2L1/MSL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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