Rabbit anti-Human GALNT4 Polyclonal Antibody | anti-GALNT4 antibody

GALNT4, NT (GALNT4, Polypeptide N-acetylgalactosaminyltransferase 4, Polypeptide GalNAc transferase 4, Protein-UDP acetylgalactosaminyltransferase 4, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4) (Azide free) (HRP)

Cat. Num. AAA6301141

• Gene Names: GALNT4; GALNACT4; GALNAC-T4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GALNT4; Polyclonal Antibody; NT (GALNT4; Polypeptide N-acetylgalactosaminyltransferase 4; Polypeptide GalNAc transferase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4) (Azide free) (HRP); UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; anti-GALNT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GALNT4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GALNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of human GALNT4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)

Flow Cytometry (FC/FACS)

(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GALNT4 antibody

Antibodies; Enzymes; Glycosyltransferase

References

O'Halloran, A.M., et al. J. Thromb. Thrombolysis 27(2):175-184(2009). Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003). Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000). Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998). Bennett, E.P., et al. Glycobiology 8(6):547-555(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 34452725
• NCBI GeneID: 8693
• NCBI Accession #: NP_003765.2
• NCBI GenBank Nucleotide #: NM_003774.4
• UniProt Accession #: Q8N4A0; O00208; B2R775; B4DMX6
• Molecular Weight: 45,702 Da
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 4
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 4
• NCBI Official Symbol: GALNT4
• NCBI Official Synonym Symbols: GALNACT4; GALNAC-T4
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 4
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 4
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT4
• UniProt Synonym Gene Names: GalNAc-T4; pp-GaNTase 4

NCBI Description

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist. [provided by RefSeq, Dec 2010]

Uniprot Description

GALNT4: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane.

Protein type: EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; perinuclear region of cytoplasm

Molecular Function: carbohydrate binding; metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: carbohydrate metabolic process; O-glycan processing