Rabbit anti-Human GALNT4 Polyclonal Antibody | anti-GALNT4 antibody

GALNT4, NT (GALNT4, Polypeptide N-acetylgalactosaminyltransferase 4, Polypeptide GalNAc transferase 4, Protein-UDP acetylgalactosaminyltransferase 4, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4) (FITC)

Cat. Num. AAA6301140

• Gene Names: GALNT4; GALNACT4; GALNAC-T4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GALNT4; Polyclonal Antibody; NT (GALNT4; Polypeptide N-acetylgalactosaminyltransferase 4; Polypeptide GalNAc transferase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4) (FITC); UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; anti-GALNT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GALNT4 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GALNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of human GALNT4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)

Flow Cytometry (FC/FACS)

(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GALNT4 antibody

Antibodies; Enzymes; Glycosyltransferase

References

O'Halloran, A.M., et al. J. Thromb. Thrombolysis 27(2):175-184(2009). Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003). Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000). Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998). Bennett, E.P., et al. Glycobiology 8(6):547-555(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 34452725
• NCBI GeneID: 8693
• NCBI Accession #: NP_003765.2
• NCBI GenBank Nucleotide #: NM_003774.4
• UniProt Accession #: Q8N4A0; O00208; B2R775; B4DMX6
• Molecular Weight: 45,702 Da
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 4
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 4
• NCBI Official Symbol: GALNT4
• NCBI Official Synonym Symbols: GALNACT4; GALNAC-T4
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 4
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 4
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT4
• UniProt Synonym Gene Names: GalNAc-T4; pp-GaNTase 4

NCBI Description

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist. [provided by RefSeq, Dec 2010]

Uniprot Description

GALNT4: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane.

Protein type: EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; perinuclear region of cytoplasm

Molecular Function: carbohydrate binding; metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: carbohydrate metabolic process; O-glycan processing