Testing Data
(Fig.1: Cell Surface staining of splenocytes using 0.5 ug of Rat IgG1 isotype control. Goat anti-Rat FITC secondary antibody was used. Purple: cells alone, Green: rat IgG1 isotype control.)
IgG1 isotype control
Rat IgG1 Isotype Control (Clone: HRPN)
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedAffinity Chromatography
Synonyms
IgG1; Monoclonal Isotype Control; Rat IgG1 Isotype Control (Clone: HRPN); Antibody to Rat IgG1; Anti-Rat IgG1 Antibody; Rat IgG1 Antibody; IgG1 isotype control
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HRPN
Purity/Purification
Purified
Affinity Chromatography
Affinity Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Applicable Applications for IgG1 isotype control
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
FACS: 0.5-2 ug/10^6 cells
Immunogen Information
Horseradish Peroxidase was used as the immunogen for this Isotype control.
Antigen
Rat IgG1
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Testing Data
(Fig.1: Cell Surface staining of splenocytes using 0.5 ug of Rat IgG1 isotype control. Goat anti-Rat FITC secondary antibody was used. Purple: cells alone, Green: rat IgG1 isotype control.)
Testing Data
(Fig.1: Cell Surface staining of splenocytes using 0.5 ug of Rat IgG1 isotype control. Goat anti-Rat FITC secondary antibody was used. Purple: cells alone, Green: rat IgG1 isotype control.)
Related Product Information for IgG1 isotype control
A Rat isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific Rat antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.
Product Categories/Family for IgG1 isotype control
Similar Products
Product Notes
The IgG1 (Catalog #AAA668903) is an Isotype Control produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). FACS: 0.5-2 ug/10^6 cells. Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG1, Monoclonal Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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