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SDS-Page

Galanin (GAL) Recombinant Protein | GAL recombinant protein

Recombinant Galanin (GAL)

Gene Names
GAL; GALN; GLNN; GMAP; GAL-GMAP
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Galanin (GAL); Recombinant Galanin (GAL); GAL recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RGWTLNSAG YLLGPHAVGN HRSFSDKNGL TSKRELRPED DMKPGSFDRS IPENNIMRTI IEFLSFLHLK EAGALDRLLDLPAAASSEDI ER
Sequence Length
123
Applicable Applications for GAL recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Arg32~Arg122 (Accession # P22466) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.8
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14.0kDa
NCBI Official Full Name
galanin peptides preproprotein
NCBI Official Synonym Full Names
galanin/GMAP prepropeptide
NCBI Official Symbol
GAL
NCBI Official Synonym Symbols
GALN; GLNN; GMAP; GAL-GMAP
NCBI Protein Information
galanin peptides; galanin prepropeptide; galanin-related peptide; galanin-message-associated peptide
UniProt Protein Name
Galanin peptides
Protein Family
UniProt Gene Name
GAL
UniProt Synonym Gene Names
GAL1; GALN; GLNN; GMAP
UniProt Entry Name
GALA_HUMAN

NCBI Description

This gene encodes a neuroendocrine peptide that is widely expressed in the central and peripheral nervous systems and also the gastrointestinal tract, pancreas, adrenal gland and urogenital tract. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Galanin has diverse physiological functions including nociception, feeding and energy homeostasis, osmotic regulation and water balance. GMAP has been demonstrated to possess antifungal activity and hypothesized to be part of the innate immune system. [provided by RefSeq, Jul 2015]

Uniprot Description

Galanin: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Belongs to the galanin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: Golgi apparatus; extracellular space; cell soma; extracellular region; secretory granule

Molecular Function: type 3 galanin receptor binding; neuropeptide hormone activity; protein binding; type 2 galanin receptor binding; type 1 galanin receptor binding

Biological Process: response to drug; nervous system development; positive regulation of cortisol secretion; positive regulation of apoptosis; positive regulation of catagen; negative regulation of lymphocyte proliferation; response to insulin stimulus; cAMP-mediated signaling; response to estrogen stimulus; neuropeptide signaling pathway; insulin secretion; regulation of glucocorticoid metabolic process; feeding behavior; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Disease: Epilepsy, Familial Temporal Lobe, 8

Research Articles on GAL

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Product Notes

The GAL gal (Catalog #AAA2018348) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Galanin (GAL) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GAL gal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RGWTLNSAG YLLGPHAVGN HRSFSDKNGL TSKRELRPED DMKPGSFDRS IPENNIMRTI IEFLSFLHLK EAGALDRLLD LPAAASSEDI ER. It is sometimes possible for the material contained within the vial of "Galanin (GAL), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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