Typical Testing Data/Standard Curve (for reference only)
Mouse Galanin peptides ELISA Kit | Gal elisa kit
Mouse Galanin peptides ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galanin peptides, During the reaction, Galanin peptides in the sample or standard competes with a fixed amount of biotin-labeled Galanin peptides for sites on a pre-coated Monoclonal antibody specific to Galanin peptides. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Galanin peptides in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a neuroendocrine peptide that is principally produced by a subpopulation of lactotrophs in the pituitary gland. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Mice lacking the encoded protein fail to lactate sufficiently due to abnormalities in the expression of prolactin and lactotroph proliferation, exhibit attenuated chronic neuropathic pain and developmental deficits in the dorsal root ganglion neurons. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature proteins. [provided by RefSeq, Jul 2016]
Uniprot Description
Galanin: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Belongs to the galanin family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; Golgi apparatus; secretory granule
Molecular Function: galanin receptor activity; galanin receptor binding; hormone activity; neuropeptide hormone activity; type 1 galanin receptor binding; type 2 galanin receptor binding; type 3 galanin receptor binding
Biological Process: cAMP-mediated signaling; feeding behavior; inflammatory response; negative regulation of cell proliferation; negative regulation of lymphocyte proliferation; nervous system development; neuropeptide signaling pathway; positive regulation of apoptosis; positive regulation of catagen; positive regulation of cortisol secretion; positive regulation of transcription from RNA polymerase II promoter; regulation of glucocorticoid metabolic process
Research Articles on Gal
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Product Notes
The Mouse Gal gal (Catalog #AAA288839) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288839 ELISA Kit recognizes Mouse Gal. It is sometimes possible for the material contained within the vial of "Galanin peptides, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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