Western Blot (WB)
(Western Blot analysis of 293T cell line GAL3ST1 expression in transfected 293T cell line usingMBS648047. Lane 1: GAL3ST1 transfected lysate (48.8kD). Lane 2: Non-transfected lysate.)
Rabbit GAL3ST1 Polyclonal Antibody | anti-GAL3ST1 antibody
GAL3ST1 (3'-phosphoadenosine-5'-phosphosulfate:GalCer Sulfotransferase, 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase, Cerebroside Sulfotransferase, Galactosylceramide Sulfotransferase, GalCer Sulfotransferase, CST) (HRP)
Gene Names
GAL3ST1; CST
Reactivity
Human, Rabbit
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GAL3ST1; Polyclonal Antibody; GAL3ST1 (3'-phosphoadenosine-5'-phosphosulfate:GalCer Sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; Cerebroside Sulfotransferase; Galactosylceramide Sulfotransferase; GalCer Sulfotransferase; CST) (HRP); anti-GAL3ST1 antibody
Host
Rabbit
Reactivity
Human, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GAL3ST1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
1745
Applicable Applications for anti-GAL3ST1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length protein corresponding to aa1-423 from human GAL3ST1(AAH14649.1).
Immunogen Sequence
MLPPQKKPWESMAKGLVLGALFTSFLLLMYSYAVPPLHAGLASTTPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKHRLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFITVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLLFFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYFKLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAALRHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRRMLTPEIQYLMDLGANLWVTKLWKFIRDFLRW
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of 293T cell line GAL3ST1 expression in transfected 293T cell line usingMBS648047. Lane 1: GAL3ST1 transfected lysate (48.8kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of 293T cell line GAL3ST1 expression in transfected 293T cell line usingMBS648047. Lane 1: GAL3ST1 transfected lysate (48.8kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-GAL3ST1 antibody
Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.
Product Categories/Family for anti-GAL3ST1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens galactose-3-O-sulfotransferase 1, mRNA
NCBI Official Synonym Full Names
galactose-3-O-sulfotransferase 1
NCBI Official Symbol
GAL3ST1
NCBI Official Synonym Symbols
CST
NCBI Protein Information
galactosylceramide sulfotransferase
Protein Family
NCBI Description
Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. This gene encodes galactosylceramide sulfotransferase, which catalyzes the sulfation of membrane glycolipids including the final step in the synthesis of sulfatide, a major lipid component of the myelin sheath. This gene exhibits elevated expression in ovarian epithelial carcinoma and the encoded enzyme exhibits elevated activity in renal cell carcinoma. Mutations in this gene may be associated with reduced insulin resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Research Articles on GAL3ST1
Similar Products
Product Notes
The GAL3ST1 (Catalog #AAA6379201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAL3ST1 (3'-phosphoadenosine-5'-phosphosulfate:GalCer Sulfotransferase, 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase, Cerebroside Sulfotransferase, Galactosylceramide Sulfotransferase, GalCer Sulfotransferase, CST) (HRP) reacts with Human, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's GAL3ST1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GAL3ST1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAL3ST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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