Western Blot (WB)
(Western Blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))
Rabbit Laminin-R Polyclonal Antibody | anti-Q215 antibody
Laminin-R (Q215) Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Laminin-R (Q215) polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Laminin-R (Q215) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Laminin-R (Q215) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)
Immunofluorescence (IF)
(IF image of MBS3004150 stained A549 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Laminin-R (Q215) # MBS3004150 (1:100) at 10ug/ml overnight at +4 degree C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC used at a 1/1000 dilution for 1h. Hoechst33342 was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(IF image of MBS3004150 stained A549 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Laminin-R (Q215) # MBS3004150 (1:100) at 10ug/ml overnight at +4 degree C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC used at a 1/1000 dilution for 1h. Hoechst33342 was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
RPSA: Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. Belongs to the ribosomal protein S2P family.
Protein type: Ribosomal
Chromosomal Location of Human Ortholog: 3p22.2
Cellular Component: membrane; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; structural constituent of ribosome; laminin binding; laminin receptor activity; ribosome binding
Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); translational termination; viral infectious cycle; rRNA export from nucleus; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; gene expression; endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); cell adhesion
Disease: Asplenia, Isolated Congenital
Research Articles on Q215
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Product Notes
The Q215 rpsa (Catalog #AAA3004150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Laminin-R (Q215) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin-R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the Q215 rpsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin-R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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