Rabbit anti-Human, Rat FZD3 Polyclonal Antibody | anti-FZD3 antibody

FZD3 Antibody

Cat. Num. AAA7043610

• Gene Names: FZD3; Fz-3
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: FZD3; Polyclonal Antibody; FZD3 Antibody; Frizzled-3; Fz-3; hFz3; anti-FZD3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 666

• Applicable Applications for anti-FZD3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Frizzled-3 protein (23-205AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68332 / sc-68333 / sc-68334 / sc-292246
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: FZD3 antibody at 1.94ug/mlLane 1: Rat heart tissueLane 2: U251 whole cell lysateLane 3: Thp-1 whole cell lysateLane 4: 293T whole cell lysateLane 5: Hela whole cell lysateLane 6: HepG-2 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 76 kDaObserved band size: 76 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue MBS7043610 at dilution 1:100)

Related Product Information for anti-FZD3 antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Required for the development of major fiber tracts in the central nervous system, including: the anterior commissure, the corpus callosum, the thalamocortical, corticothalamic and nigrostriatal tracts, the corticospinal tract, the fasciculus retroflexus, the mammillothalamic tract, the medial lemniscus, and ascending fiber tracts from the spinal cord to the brain. In the peripheral nervous system, controls axon growth in distinct populations of cranial and spinal motor neurons, including the facial branchimotor nerve, the hypoglossal nerve, the phrenic nerve, and motor nerves innervating dorsal limbs. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 8393378
• NCBI GeneID: 7976
• NCBI Accession #: NP_059108.1
• NCBI GenBank Nucleotide #: NM_017412.3
• UniProt Accession #: Q9NPG1; A8K615
• Molecular Weight: – Da
• NCBI Official Full Name: frizzled-3
• NCBI Official Synonym Full Names: frizzled class receptor 3
• NCBI Official Symbol: FZD3
• NCBI Official Synonym Symbols: Fz-3
• NCBI Protein Information: frizzled-3
• UniProt Protein Name: Frizzled-3
• UniProt Gene Name: FZD3
• UniProt Synonym Gene Names: Fz-3; hFz3
• UniProt Entry Name: FZD3_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Uniprot Description

FZD3: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; PDZ domain binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of mitotic cell cycle, embryonic; neuron differentiation; positive regulation of neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway