Rabbit anti-Mouse, Human FZD3 / Frizzled 3 Polyclonal Antibody | anti-FZD3 antibody

Anti-FZD3 / Frizzled 3 Antibody (aa481-530) IHC-plus

Cat. Num. AAA2400195

• Gene Names: FZD3; Fz-3
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: FZD3 / Frizzled 3; Polyclonal Antibody; Anti-FZD3 / Frizzled 3 Antibody (aa481-530) IHC-plus; Rabbit Polyclonal (IgG) to Human FZD3 / Frizzled 3; Human FZD3 / Frizzled 3; Frizzled family receptor 3; Frizzled 3; HFz3; Frizzled homolog 3; Fz-3; Wnt receptor frizzled-3; Frizzled-3; anti-FZD3 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FZD3 Antibody detects endogenous levels of total FZD3 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 666

• Applicable Applications for anti-FZD3 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000); IF (1:100 - 1:500); IHC-P (20 ug/ml); WB (1:500 - 1:1000)
• Immunogen: FZD3 / Frizzled 3 antibody was raised against synthetic peptide from human FZD3 (aa481-530).
• Immunogen Description: Synthetic peptide from human FZD3 (aa481-530).
• Antigen Modification: aa481-530
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-FZD3 / Frizzled 3 antibody IHC staining of human brain, oligodendroglia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using FZD3 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HUVEC cells, using FZD3 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-FZD3 antibody

FZD3 Antibody, Frizzled family receptor 3 Antibody, Frizzled 3 Antibody, HFz3 Antibody, Frizzled homolog 3 Antibody, Fz-3 Antibody, Wnt receptor frizzled-3 Antibody, Frizzled-3 Antibody Description: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.

NCBI and Uniprot Product Information

• NCBI GI #: 8393378
• NCBI GeneID: 7976
• NCBI Accession #: NP_059108.1
• NCBI GenBank Nucleotide #: NM_017412.3
• UniProt Accession #: Q9NPG1; A8K615
• Molecular Weight: – Da
• NCBI Official Full Name: frizzled-3
• NCBI Official Synonym Full Names: frizzled class receptor 3
• NCBI Official Symbol: FZD3
• NCBI Official Synonym Symbols: Fz-3
• NCBI Protein Information: frizzled-3
• UniProt Protein Name: Frizzled-3
• UniProt Gene Name: FZD3
• UniProt Synonym Gene Names: Fz-3; hFz3
• UniProt Entry Name: FZD3_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Uniprot Description

FZD3: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, Fz/Smo family; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: apical plasma membrane; axon; cell soma; cell surface; cytoplasm; dendrite; filopodium tip; integral to membrane; lateral plasma membrane; plasma membrane; presynaptic active zone

Molecular Function: G-protein coupled receptor activity; PDZ domain binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cell proliferation in midbrain; establishment of planar polarity; G-protein coupled receptor protein signaling pathway; hair follicle development; inner ear morphogenesis; negative regulation of mitotic cell cycle, embryonic; neural tube closure; neuron differentiation; neuron migration; positive regulation of neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin