Rabbit anti-Human FZD1 Polyclonal Antibody | anti-FZD1 antibody

FZD1, CT (Frizzled-1, Fz-1, hFz1, FzE1) (HRP)

Cat. Num. AAA6476933

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: FZD1; Polyclonal Antibody; CT (Frizzled-1; Fz-1; hFz1; FzE1) (HRP); anti-FZD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FZD1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FZD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human FZD1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-FZD1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).FZD1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-FZD1 antibody

Antibodies; Wnt Proteins

References

1. Quelard, D., (er) PLoS ONE 3(4), E1878 (2008). 2. Hardie, W.D., Am. J. Respir. Cell Mol. Biol. 37(3), 309-321 (2007). 3. Yang, L., J. Dermatol. Sci. 42(2), 111-119 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4503825
• NCBI GeneID: 8321
• NCBI Accession #: NP_003496
• NCBI GenBank Nucleotide #: NM_003505
• UniProt Accession #: Q9UP38; O94815; Q549T8; A4D1E8
• Molecular Weight: 71,158 Da
• NCBI Official Full Name: frizzled-1
• NCBI Official Synonym Full Names: frizzled class receptor 1
• NCBI Official Symbol: FZD1
• NCBI Protein Information: frizzled-1; Wnt receptor; frizzled 1, seven transmembrane spanning receptor; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; fz-1; fzE1; hFz1
• UniProt Protein Name: Frizzled-1
• Protein Family: Frizzled
• UniProt Gene Name: FZD1
• UniProt Synonym Gene Names: Fz-1; hFz1
• UniProt Entry Name: FZD1_HUMAN

Uniprot Description

FZD1: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; GPCR, Fz/Smo family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: focal adhesion; cell surface; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; frizzled binding; receptor binding; PDZ domain binding

Biological Process: neuron differentiation; response to drug; G-protein coupled receptor protein signaling pathway; cell-cell signaling; epithelial cell differentiation; positive regulation of transcription, DNA-dependent; hard palate development; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway