Rabbit anti-Rat Fibronectin (FN) Polyclonal Antibody | anti-FN antibody

Polyclonal Antibody to Fibronectin (FN)

Cat. Num. AAA2004823

• Gene Names: Fn1; fn-1; FIBNEC
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Fibronectin (FN); Polyclonal Antibody; Polyclonal Antibody to Fibronectin (FN); anti-FN antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.71mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS- SNGEPC VLPFHYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHAVLVQT RGGNSNGALC HFPFLYSNRN YSDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDL GHMMRCTCVG NGRGQWACIP YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPI
• Sequence Length: 2481

• Applicable Applications for anti-FN antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:50-400; Immunocytochemistry in formalin fixed cells: 1:50-500; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Immunohistochemistry in paraffin section: 1:10-100; Enzyme-linked Immunosorbent Assay: 1:100-200; Optimal working dilutions must be determined by end user.
• Immunogen: FN, Rat
• Immunogen Information: Recombinant FN (Ser355~Ile558) with N-terminal His-Tag expressed in E.coli.
• Accession No.: RPA037Ra01
• UOM: 100ug
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2035704
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant Fibronectin, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Heart Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Testis Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 120178
• NCBI GeneID: 25661
• UniProt Accession #: P04937; Q6LDX9
• Molecular Weight: 259,350 Da
• NCBI Official Full Name: Fibronectin
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: fn-1; FIBNEC
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN

NCBI Description

extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: apical plasma membrane; basal lamina; basement membrane; endoplasmic reticulum-Golgi intermediate compartment; extracellular matrix; extracellular region; extracellular space; fibrinogen complex; proteinaceous extracellular matrix

Molecular Function: chaperone binding; enzyme binding; extracellular matrix structural constituent; heparin binding; identical protein binding; integrin binding; mercury ion binding; protease activator activity; protease binding; protein binding; protein C-terminus binding; receptor binding

Biological Process: acute-phase response; angiogenesis; calcium-independent cell-matrix adhesion; cell activation; cell adhesion; cell-matrix adhesion; cell-substrate junction assembly; extracellular matrix organization; glial cell migration; integrin activation; interaction with other organism via secreted substance during symbiotic interaction; interaction with symbiont; negative regulation of apoptosis; ossification; peptide cross-linking; positive regulation of axon extension; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of fibroblast proliferation; regulation of cell shape; regulation of protein amino acid phosphorylation; response to activity; response to drug; response to glucocorticoid stimulus; response to iron ion; response to ozone; response to wounding; wound healing