Rabbit anti-Human FUT8 Polyclonal Antibody | anti-FUT8 antibody

FUT8, ID (FUT8, Alpha-(1,6)-fucosyltransferase, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase, GDP-fucose-glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase) (Azide free) (HRP)

Cat. Num. AAA6300437

• Gene Names: FUT8; CDGF; CDGF1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FUT8; Polyclonal Antibody; ID (FUT8; Alpha-(1; 6)-fucosyltransferase; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1; 6-fucosyltransferase; GDP-fucose-glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase) (Azide free) (HRP); GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; anti-FUT8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FUT8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the Central region of human FUT8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FUT8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).)

Immunohistochemistry (IHC)

(FUT8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FUT8 antibody

This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.

Product Categories/Family for anti-FUT8 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Wang, X., et al. J. Biochem. 145(5):643-651(2009). Kudo, T., et al. Mol. Cancer 6, 32 (2007): Ihara, H., et al. Glycobiology 16(4):333-342(2006). Ito, Y., et al. Cancer Lett. 200(2):167-172(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 30410724
• NCBI GeneID: 2530
• UniProt Accession #: Q9BYC5
• NCBI Official Synonym Full Names: fucosyltransferase 8
• NCBI Official Symbol: FUT8
• NCBI Official Synonym Symbols: CDGF; CDGF1
• NCBI Protein Information: alpha-(1,6)-fucosyltransferase
• UniProt Protein Name: Alpha-(1,6)-fucosyltransferase
• Protein Family: Probable fucosyltransferase
• UniProt Gene Name: FUT8
• UniProt Synonym Gene Names: Alpha1-6FucT
• UniProt Entry Name: FUT8_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

Uniprot Description

FUT8: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. Belongs to the glycosyltransferase 23 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Glycan Metabolism - N-glycan biosynthesis; Glycan Metabolism - keratan sulfate biosynthesis; Transferase; Membrane protein, integral; EC 2.4.1.68

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: Golgi membrane; Golgi apparatus; membrane; cytoplasm; integral to membrane

Molecular Function: glycoprotein 6-alpha-L-fucosyltransferase activity; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; cell migration; receptor metabolic process; in utero embryonic development; oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation; respiratory gaseous exchange; post-translational protein modification; GDP-L-fucose metabolic process; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; protein amino acid glycosylation in Golgi; protein amino acid N-linked glycosylation via asparagine; L-fucose catabolic process; N-glycan processing