Rabbit anti-Human FUT8 Polyclonal Antibody | anti-FUT8 antibody

FUT8, ID (FUT8, Alpha-(1,6)-fucosyltransferase, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase, GDP-fucose-glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase)

Cat. Num. AAA648567

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FUT8; Polyclonal Antibody; ID (FUT8; Alpha-(1; 6)-fucosyltransferase; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1; 6-fucosyltransferase; GDP-fucose-glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase); Anti -FUT8; anti-FUT8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-FUT8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the Central region of human FUT8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FUT8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).)

Immunohistochemistry (IHC)

(FUT8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FUT8 antibody

This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.

Product Categories/Family for anti-FUT8 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 148745747
• NCBI GeneID: 2530
• UniProt Accession #: Q9BYC5; O00235; Q9BYC6; Q9P2U5; Q9P2U6; B4DFS7; G3V5N0
• Molecular Weight: 66,516 Da
• NCBI Official Full Name: FUT8 protein
• NCBI Official Synonym Full Names: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• NCBI Official Symbol: FUT8
• NCBI Protein Information: alpha-(1,6)-fucosyltransferase; alpha-(1,6)-fucosyltransferase; alpha1-6FucT; glycoprotein 6-alpha-L-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
• UniProt Protein Name: Alpha-(1,6)-fucosyltransferase
• Protein Family: Probable fucosyltransferase
• UniProt Gene Name: FUT8
• UniProt Synonym Gene Names: Alpha1-6FucT
• UniProt Entry Name: FUT8_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

Uniprot Description

Function: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. Ref.1

Catalytic activity: GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein

By similarity. Note: Membrane-bound form in trans cisternae of Golgi

By similarity.

Sequence similarities: Belongs to the glycosyltransferase 23 family.Contains 1 GT23 domain.Contains 1 SH3 domain.