Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat FURIN/PCSK3 Polyclonal Antibody | anti-FURIN antibody
Goat anti-FURIN/PCSK3 Antibody
Western Blot: In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx.110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.
Protein type: Protease; Membrane protein, integral; Vesicle; Cell surface; EC 3.4.21.75
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular space; Golgi cisterna; Golgi lumen; Golgi membrane; integral to membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle
Molecular Function: endopeptidase activity; metal ion binding; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity
Biological Process: aging; cell proliferation; cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of nerve growth factor production; negative regulation of transforming growth factor-beta1 production; nerve growth factor processing; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of cell migration; positive regulation of membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; protein processing; regulation of endopeptidase activity; regulation of low-density lipoprotein receptor biosynthetic process; regulation of protein catabolic process; secretion by cell; signal peptide processing; transforming growth factor beta receptor signaling pathway; viral infectious cycle; viral protein processing
Research Articles on FURIN
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Product Notes
The FURIN furin (Catalog #AAA423346) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FURIN/PCSK3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FURIN/PCSK3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx.110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml. Researchers should empirically determine the suitability of the FURIN furin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RTKRDVYQEP TDP. It is sometimes possible for the material contained within the vial of "FURIN/PCSK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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