Western Blot (WB)
(Western blot analysis of Calponin-3 Antibody (N-term) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Calponin-3 Polyclonal Antibody | anti-CNN3 antibody
Calponin-3, NT (Calponin 3, Calponin, Acidic Isoform, CNN3)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(Western blot analysis of Calponin-3 Antibody (N-term) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of Calponin-3 Antibody (N-term) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Calponin-3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Calponin-3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Calponin-3 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Calponin-3 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]
Uniprot Description
calponin 3: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1p22-p21
Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton
Molecular Function: calmodulin binding; actin binding
Biological Process: epithelial cell differentiation; actomyosin structure organization and biogenesis
Research Articles on CNN3
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Product Notes
The CNN3 cnn3 (Catalog #AAA629871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calponin-3, NT (Calponin 3, Calponin, Acidic Isoform, CNN3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calponin-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the CNN3 cnn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calponin-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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