Human Furin ELISA Kit | FUR elisa kit

Human Furin ELISA Kit

Cat. Num. AAA765166

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Reactivity: Human
• Synonyms: Furin; Human Furin ELISA Kit; Furin/FUR; FUR elisa kit

• Reactivity: Human
• Sequence Length: 794

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FUR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human Furinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human Furin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Furin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman Furincan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 577019580
• NCBI GeneID: 5045
• NCBI Accession #: NP_001276753.1
• NCBI GenBank Nucleotide #: NM_001289824.1
• Molecular Weight: 86,678 Da
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin, paired basic amino acid cleaving enzyme
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Furin
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Vesicle; Membrane protein, integral; EC 3.4.21.75; Cell surface; Protease

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular space; Golgi cisterna; Golgi lumen; Golgi membrane; integral to membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function: endopeptidase activity; metal ion binding; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity

Biological Process: aging; cell proliferation; cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of nerve growth factor production; negative regulation of transforming growth factor-beta1 production; nerve growth factor processing; nerve growth factor production; nerve growth factor receptor signaling pathway; Notch signaling pathway; peptide biosynthetic process; peptide hormone processing; peptidyl-glutamic acid carboxylation; positive regulation of cell migration; positive regulation of membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; post-translational protein modification; protein processing; regulation of endopeptidase activity; regulation of low-density lipoprotein receptor biosynthetic process; regulation of protein catabolic process; secretion by cell; signal peptide processing; transforming growth factor beta receptor signaling pathway; viral infectious cycle; viral protein processing; viral reproduction; virus assembly