Goat anti-Human FTO Polyclonal Antibody | anti-fto antibody

FTO (Fatso, Fat Mass and Obesity-associated Protein, Protein Fatso)

Cat. Num. AAA619275

• Gene Names: fto; MGC81641
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: FTO; Polyclonal Antibody; FTO (Fatso; Fat Mass and Obesity-associated Protein; Protein Fatso); Anti -FTO (Fatso; anti-fto antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes FTO. Species Sequence Homology: human, rat, canine, porcine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

• Applicable Applications for anti-fto antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:128,000
• Immunogen: Synthetic peptide corresponding to QQKPDCRPYWEKDD from the Internal region of FTO (NP_036066.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(EB08564 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-fto antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 148222349
• NCBI GeneID: 447305
• NCBI Accession #: NP_001087481.1
• NCBI GenBank Nucleotide #: NM_001094012.1
• UniProt Accession #: Q68F54
• Molecular Weight: 58,527 Da
• NCBI Official Full Name: alpha-ketoglutarate-dependent dioxygenase FTO
• NCBI Official Synonym Full Names: fat mass and obesity associated<
• NCBI Official Symbol: fto
• NCBI Official Synonym Symbols: MGC81641
• NCBI Protein Information: alpha-ketoglutarate-dependent dioxygenase FTO; protein fto
• UniProt Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Protein Family: Alpha-ketoglutarate-dependent dioxygenase
• UniProt Gene Name: fto
• UniProt Entry Name: FTO_XENLA

Uniprot Description

Function: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. May contribute to the regulation of the global metabolic rate, energy expenditure and energy homeostasis

Cofactor: Binds 1 Fe2+ ion per subunit

Subcellular location: Nucleus

Domain: The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases

Sequence similarities: Belongs to the fto family.