Chemiluminescence
(EB08943 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
anti-Rat DPM1 Polyclonal Antibody | anti-DPM1 antibody
Anti - DPM1 (Dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, RP23-391M18.1, AI118379, AI194292, OTTMUSP00000017164, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1)
Reactivity
Rat
Applications
Western Blot
Purity
Purified by affinity chromatography.
Synonyms
DPM1; Polyclonal Antibody; Anti - DPM1 (Dolichyl-phosphate mannosyltransferase polypeptide 1; catalytic subunit; RP23-391M18.1; AI118379; AI194292; OTTMUSP00000017164; dolichol monophosphate mannose synthase; dolichyl-phosphate mannosyltransferase polypeptide 1); DPM1 (Dolichyl-phosphate mannosyltransferase polypeptide 1; anti-DPM1 antibody
Host
Host: Goat
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat DPM1. Sequence Homology: mouse.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Concentration
~0.5mg/ml (varies by lot)
Applicable Applications for anti-DPM1 antibody
Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: ELISA: 1:64,000
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:64,000
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide corresponding to PQGRSSRQDKYS-C from the N-Terminus of rat DPM1 (NP_034202.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
(EB08943 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Chemiluminescence
(EB08943 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Related Product Information for anti-DPM1 antibody
Dolichol-phosphate mannose (DPM1) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Defective surface expression of GPI-anchored proteins occurs when there is a lack of DPM1. DPM1 is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
Product Categories/Family for anti-DPM1 antibody
References
• 1. Ashida H, Maeda Y, Kinoshita T. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3. J Biol Chem. 2006 Jan 13;281(2):896-904. PMID: 16280320.
Similar Products
Product Notes
The DPM1 (Catalog #AAA622469) is an Antibody produced from Host: Goat Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - DPM1 (Dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, RP23-391M18.1, AI118379, AI194292, OTTMUSP00000017164, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DPM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: ELISA: 1:64,000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the DPM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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