Immunofluorescence (IF)
(Fluorescent image of B-3 cell stained with FOXD1 Antibody (N-term).B-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).FOXD1 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human FOXD1 Polyclonal Antibody | anti-FOXD1 antibody
FOXD1, NT (FOXD1, FKHL8, FREAC4, Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4) (Biotin)
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of B-3 cell stained with FOXD1 Antibody (N-term).B-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).FOXD1 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of B-3 cell stained with FOXD1 Antibody (N-term).B-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).FOXD1 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(FOXD1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXD1 antibody detected the FOXD1 protein (arrow).)
Western Blot (WB)
(FOXD1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXD1 antibody detected the FOXD1 protein (arrow).)
Immunohistochemistry (IHC)
(FOXD1 Antibody (N-term) (AP14388a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FOXD1 Antibody (N-term) (AP14388a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Studies of the orthologous mouse protein indicate that it functions in kidney development by promoting nephron progenitor differentiation, and it also functions in the development of the retina and optic chiasm. It may also regulate inflammatory reactions and prevent autoimmunity. [provided by RefSeq, Apr 2014]
Uniprot Description
FOXD1: Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly.
Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; DNA bending activity; transcription factor activity
Biological Process: axon guidance; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of BMP signaling pathway
Research Articles on FOXD1
Similar Products
Product Notes
The FOXD1 foxd1 (Catalog #AAA6299742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOXD1, NT (FOXD1, FKHL8, FREAC4, Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXD1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FOXD1 foxd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
