Flow Cytometry (FC/FACS)
(Fig.1: GM-0105. BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human IL17RB (filled red curve), or an irrelevant protein (control transfectant, black curve). Binding of BFI-7C7 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with human IL17RB.)
Rat Interleukin-17 Receptor B (IL17RB) Monoclonal Antibody | anti-IL17RB antibody
Rat anti-human Interleukin-17 Receptor B (IL17RB) monoclonal antibody
ELISA: 1:200 - 1:400
C-ELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentratio.
Long term: -20°C.
Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
(Fig.1: GM-0105. BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human IL17RB (filled red curve), or an irrelevant protein (control transfectant, black curve). Binding of BFI-7C7 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with human IL17RB.)
Flow Cytometry (FC/FACS)
(Fig.1: GM-0105. BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human IL17RB (filled red curve), or an irrelevant protein (control transfectant, black curve). Binding of BFI-7C7 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with human IL17RB.)
Testing Data
(Fig.2: CGE analysis of purified BFI-7C7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BFI-7C7 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Testing Data
(Fig.2: CGE analysis of purified BFI-7C7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BFI-7C7 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
The IL17RB protein is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17A or IL17C (1, 2). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E (3,4). The expression of the rat counterpart of this gene was found to be significantly up-regulated during intestinal inflammation, which suggested the immunoregulatory activity of this receptor. This antibody is recommended for applications under non-denaturing conditions.
2. Reynolds JM et al. (2015). Interleukin-17B antagonizes Interleukin-25-mediated mucosal inflammation. Immunity 42(4):692-703.
3. Maezawa Y et al. (2006). Involvement of TNF receptor-associated factor 6 in IL-25 receptor signalling. J. Immunol. 176(2):1013-1018.
4. Hata K et al. (2002). IL-17 stimulates inflammatory responses via NF-kappaB and MAP kinase pathways in human colonic myofibroblasts. Am. J. Physiol. Gastrointest. Liver Physiol. 282(6):G1035-1044.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E, but does not bind to IL17 and IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. The expression of the rat counterpart of this gene was found to be significantly up-regulated during intestinal inflammation, which suggested the immunoregulatory activity of this receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17RB: Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: cell surface; cytoplasm; extracellular region; integral to plasma membrane; membrane; plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-17 receptor activity
Biological Process: cytokine and chemokine mediated signaling pathway; defense response; positive regulation of inflammatory response; regulation of cell growth
Research Articles on IL17RB
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Product Notes
The IL17RB il17rb (Catalog #AAA468049) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin-17 Receptor B (IL17RB) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), C-ELISA (EIA). Flow Cytometry: 1.2 ug/10^6 cells ELISA: 1:200 - 1:400 C-ELISA: 1:200 - 1:400 For each application a titration should be performed to determine the optimal concentratio. Researchers should empirically determine the suitability of the IL17RB il17rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin-17 Receptor B (IL17RB), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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