Immunohistochemistry (IHC)
(Anti-FOXA2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Mouse anti-Human FOXA2 Monoclonal Antibody | anti-FOXA2 antibody
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human FOXA2
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Immunohistochemistry (IHC)
(Anti-FOXA2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-FOXA2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(FOXA2 monoclonal antibody clone 2F12 Western blot of FOXA2 expression in HepG2.)
Western Blot (WB)
(FOXA2 monoclonal antibody clone 2F12 Western blot of FOXA2 expression in HepG2.)
ELISA (EIA)
(Detection limit for recombinant GST tagged FOXA2 is approximately 0.1 ng/ml as a capture antibody.)
ELISA (EIA)
(Detection limit for recombinant GST tagged FOXA2 is approximately 0.1 ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
FOXA2: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. Binds DNA as a monomer. Binds TLE1. Interacts with FOXA1 and FOXA3. Interacts with PRKDC. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 20p11
Cellular Component: cytoplasm; nucleus
Molecular Function: protein domain specific binding; DNA binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; adult locomotory behavior; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter by glucose; cell fate specification; chromatin modification; somite rostral/caudal axis specification; endocrine pancreas development; negative regulation of glucokinase activity; regulation of transcription from RNA polymerase II promoter; cell differentiation in hindbrain; negative regulation of neuron differentiation; positive regulation of embryonic development; ectoderm formation; regulation of steroid metabolic process; regulation of blood coagulation; neuron fate specification; positive regulation of transcription from RNA polymerase III promoter; positive regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell development; dorsoventral neural tube patterning
Research Articles on FOXA2
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Product Notes
The FOXA2 foxa2 (Catalog #AAA245729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human FOXA2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXA2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. Researchers should empirically determine the suitability of the FOXA2 foxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXA2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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