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Dot Blot (DB) (Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Rabbit anti-Human FOS Polyclonal Antibody | anti-FOS antibody

FOS, Phosphorylated (Thr232) (Proto-oncogene c-Fos, Cellular Oncogene Fos, G0/G1 Switch Regulatory Protein 7, G0S7) (AP)

Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
ELISA, Dot Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
FOS; Polyclonal Antibody; Phosphorylated (Thr232) (Proto-oncogene c-Fos; Cellular Oncogene Fos; G0/G1 Switch Regulatory Protein 7; G0S7) (AP); anti-FOS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FOS when phosphorylated at Thr232.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-FOS antibody
ELISA (EIA), Dot Blot (DB)
Application Notes
ELISA: 1:1,000
Dot Blot: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr232 of human FOS (KLH).
Conjugate
AP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Dot Blot (DB) (Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Product Categories/Family for anti-FOS antibody
References
1. Gensch, E., et al., J. Biol. Chem. 279(37):39085-39093 (2004). 2. Myllykangas, S., et al., Genes Chromosomes Cancer 40(4):334-341 (2004). 3. Wu, M.Y., et al., World J. Gastroenterol. 10(4):476-480 (2004). 4. Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003). 5. Lavezzi, A.M., et al., Pathol. Int. 53(11):769-774 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,301 Da
NCBI Official Full Name
proto-oncogene c-Fos
NCBI Official Synonym Full Names
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol
FOS
NCBI Official Synonym Symbols
p55; AP-1; C-FOS
NCBI Protein Information
proto-oncogene c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); G0/G1 switch regulatory protein 7; activator protein 1; cellular oncogene c-fos
UniProt Protein Name
Proto-oncogene c-Fos
Protein Family
UniProt Gene Name
FOS
UniProt Synonym Gene Names
G0S7
UniProt Entry Name
FOS_HUMAN

Uniprot Description

Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; nucleus; cytosol

Molecular Function: protein binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; conditioned taste aversion; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; aging; response to corticosterone stimulus; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; cellular response to hormone stimulus; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to cold; response to progesterone stimulus

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Product Notes

The FOS fos (Catalog #AAA6476775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOS, Phosphorylated (Thr232) (Proto-oncogene c-Fos, Cellular Oncogene Fos, G0/G1 Switch Regulatory Protein 7, G0S7) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot Blot (DB). ELISA: 1:1,000 Dot Blot: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FOS fos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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