ULBP1 / RAET1 / N2DL1 recombinant protein

Recombinant Human ULBP1 / RAET1 / N2DL1 Protein (His & Fc tag)

Cat. Num. AAA2546148

• Gene Names: ULBP1; N2DL-1; RAET1I; NKG2DL1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ULBP1 / RAET1 / N2DL1; Recombinant Human ULBP1 / RAET1 / N2DL1 Protein (His & Fc tag); RAET1I; ULBP1; N2DL1; ULBP1 / RAET1 / N2DL1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 244

• Application Notes: The mature form of recombinant human ULBP1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 439 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhULBP1/Fc monomer is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gly 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ULBP1 / RAET1 / N2DL1 recombinant protein

Background: UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins, and have tissue-specific expressions. ULBP1, also known as RAET1I and NKG2DL1, together with at least ULBP 2 and 3, are well-known ligands for NKG2D, and activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver and bone marrow, as well as some tumor cells. As an unconventional member of the MHC class I family, ULBP1 function in immune responses, especially in cancer and infectious diseases. Unlike other ULBP members, ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor, and thus might escape the immune surveillance. Furthermore, UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.

Description: A DNA sequence encoding the mature form of human ULBP1 (NP_079494.1) (Met 1-Gly 216) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13376826
• NCBI GeneID: 80329
• NCBI Accession #: NP_079494.1
• NCBI GenBank Nucleotide #: NM_025218.3
• UniProt Accession #: Q9BZM6; Q5VY81; Q8IZW3; Q8IZX6
• Molecular Weight: 27,997 Da
• NCBI Official Full Name: NKG2D ligand 1 isoform 1
• NCBI Official Synonym Full Names: UL16 binding protein 1
• NCBI Official Symbol: ULBP1
• NCBI Official Synonym Symbols: N2DL-1; RAET1I; NKG2DL1
• NCBI Protein Information: NKG2D ligand 1
• UniProt Protein Name: NKG2D ligand 1
• UniProt Gene Name: ULBP1
• UniProt Synonym Gene Names: N2DL1; RAET1I; N2DL-1; NKG2DL1

NCBI Description

The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Apoptosis; Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity; regulation of immune response