Rabbit anti-Human Formin-like Protein 2 Polyclonal Antibody | anti-FMNL2 antibody

Formin-like Protein 2 (FMNL2, Formin Homology 2 Domain-containing Protein 2, FHOD2, FLJ37546, KIAA1902)

Cat. Num. AAA646359

• Gene Names: FMNL2; FHOD2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Formin-like Protein 2; Polyclonal Antibody; Formin-like Protein 2 (FMNL2; Formin Homology 2 Domain-containing Protein 2; FHOD2; FLJ37546; KIAA1902); Anti -Formin-like Protein 2 (FMNL2; anti-FMNL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FMNL2. Species sequence homology: Bovine, chimpanzee, monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-FMNL2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5-10ug/ml
• Immunogen: Synthetic peptide corresponding to aa1-50 from human FMNL2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with FMNL2 antibody at 5-10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-FMNL2 antibody

Formin-like protein 2 is a cytoplasmic phosphoprotein belonging to the formin homology domain containing protein family and has a DAD (diaphanous autoregulatory domain), FH2 (formin homology 2) domain, and GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. The exact function of FMNL2 is yet to be known but reports suggest that it may have a role in the Wnt signaling pathway. Formin-related proteins are also known to play an important role in morphogenesis, cytokinesis and cell polarity. Several alternatively spliced transcript variants have been described but their full-length nature is not yet determined. It is also known to be associated with a diffuse-type gastric cancer, breast cancer, chondrosarcoma, melanoma, and glioblastoma. FMNL2 might be implicated in polarity control, invasion, migration or metastasis through actin filament nucleation and may be regulated by Rho family GTPase.

Product Categories/Family for anti-FMNL2 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 52485606
• NCBI GeneID: 114793
• NCBI Accession #: NP_443137.2
• NCBI GenBank Nucleotide #: NM_052905.3
• UniProt Accession #: Q96PY5; Q14CC9; Q4ZG52; Q8N3E0; B2RZH5
• Molecular Weight: 123,321 Da
• NCBI Official Full Name: formin-like protein 2
• NCBI Official Synonym Full Names: formin-like 2
• NCBI Official Symbol: FMNL2
• NCBI Official Synonym Symbols: FHOD2
• NCBI Protein Information: formin-like protein 2; formin homology 2 domain containing 2; formin homology 2 domain-containing protein 2
• UniProt Protein Name: Formin-like protein 2
• Protein Family: Formin-like protein
• UniProt Gene Name: FMNL2
• UniProt Synonym Gene Names: FHOD2; KIAA1902
• UniProt Entry Name: FMNL2_HUMAN

NCBI Description

This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis, cytokinesis, and cell polarity. Alternatively spliced transcript variants encoding different isoforms have been described but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics. Ref.10

Subcellular location: Cytoplasm

By similarity.

Domain: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments

By similarity.

Sequence similarities: Belongs to the formin homology family.Contains 1 DAD (diaphanous autoregulatory) domain.Contains 1 FH2 (formin homology 2) domain.Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

Sequence caution: The sequence AAI13879.1 differs from that shown. Reason: Aberrant splicing.The sequence AAI14439.1 differs from that shown. Reason: Aberrant splicing.The sequence AAX88959.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB67795.1 differs from that shown. Reason: Erroneous initiation.