SIRT3 recombinant protein

SIRT3 recombinant protein

Cat. Num. AAA515315

• Gene Names: SIRT3; SIR2L3
• Applications: Western Blot
• Synonyms: SIRT3; SIRT3 recombinant protein; SIR2L3; Sirtuin 3

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2919

• Applicable Applications for SIRT3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Full-length recombinant human SIRT3 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for SIRT3 recombinant protein

Full-length recombinant human SIRT3 was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: SIRT3 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT3 being a member of class I. SIRT3 contains an N-terminal mitochondrial targeting signal and a central catalytic domain and localizes in the nuclei and mitochondria (1). SIRT3 has NAD(+)-dependent histone deacetylase activity with specificity for lys16 of H4 and, to a lesser extent, lys9 of H3. SIRT3 can repress transcription of a target gene in vivo when recruited to its promoter. Full-length SIRT3 is transported from the nucleus to the mitochondria during cell stress conditions (2).

Product Categories/Family for SIRT3 recombinant protein

Deacetylases/Demethylase Proteins; Signaling Proteins - Deacetylase/Demethylase Proteins

References

1. Onyango, P. et al: SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria. Proc. Nat. Acad. Sci. 99: 13653-13658, 2002.
2. Scher, M. B. et al: SirT3 is a nuclear NAD(+)-dependent histone deacetylase that translocates to the mitochondria upon cellular stress. Genes Dev. 21: 920-928, 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 157671924
• NCBI GeneID: 23410
• NCBI Accession #: NP_036371.1
• NCBI GenBank Nucleotide #: NM_012239
• UniProt Accession #: Q9NTG7; Q9Y6E8; B7Z5U6
• Molecular Weight: ~66 kDa
• NCBI Official Full Name: Homo sapiens sirtuin 3 (SIRT3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: sirtuin 3
• NCBI Official Symbol: SIRT3
• NCBI Official Synonym Symbols: SIR2L3
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: SIRT3
• UniProt Synonym Gene Names: SIR2L3; hSIRT3
• UniProt Entry Name: SIR3_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. Ref.9 Ref.11 Ref.12 Ref.14

Catalytic activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.6 Ref.7 Ref.9

Cofactor: Binds 1 zinc ion per subunit.

Subunit structure: Interacts with NDUFA9, ACSS1, IDH2 and GDH. Ref.9 Ref.12

Subcellular location: Mitochondrion matrix Ref.6 Ref.7 Ref.8 Ref.10.

Tissue specificity: Widely expressed. Ref.1 Ref.7

Post-translational modification: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

Miscellaneous: Has some ability to deacetylate histones in vitro, but seeing its subcellular location, this is unlikely in vivo.

Sequence similarities: Belongs to the sirtuin family. Class I subfamily.Contains 1 deacetylase sirtuin-type domain.

Biophysicochemical propertiesKinetic parameters:KM=600 µM for NAD Ref.14