Immunofluorescence (IF)
(Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9. 0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-66 at 1/50 dilution for 10 hours at 4 degree C and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.)
Rabbit anti-Zebrafish FNDC3A Polyclonal Antibody | anti-FNDC3A antibody
FNDC3A Polyclonal Antibody
Reactivity
Zebrafish
Applications
Immunofluorescence, Immunohistochemistry
Purity
Protein affinity purified.
Synonyms
FNDC3A; Polyclonal Antibody; FNDC3A Polyclonal Antibody; fndc7a; Fibronectin type III domain containing protein 3a; FNDC3; HUGO; KIAA0970 antibody; anti-FNDC3A antibody
Host
Rabbit
Reactivity
Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
FNDC3A polyclonal antibody detects endogenous levels of FNDC3A protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-FNDC3A antibody
Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein within Zebrafish FNDC3A aa 100-310.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9. 0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-66 at 1/50 dilution for 10 hours at 4 degree C and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.)
Immunofluorescence (IF)
(Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9. 0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-66 at 1/50 dilution for 10 hours at 4 degree C and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8. 0-8. 4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-66 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8. 0-8. 4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ER1803-66 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
Related Product Information for anti-FNDC3A antibody
Fibronectin type III domain containing 7, or, FNDC7, is a human gene. Other names include: Fibronectin type III domain-containing protein 7. The encoded protein has an amino acid length of 733 and a mass of 78.2 kDa. Other isoforms may exist. This gene shares homology with other species, including: Mouse, Rat, Zebrafish, Frog, Chicken.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
67kDa
Protein Family
Similar Products
Product Notes
The FNDC3A (Catalog #AAA3012705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FNDC3A Polyclonal Antibody reacts with Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's FNDC3A can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC). IF: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FNDC3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FNDC3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
