Rabbit Fli-1 Polyclonal Antibody | anti-Fli-1 antibody

Fli-1 (R319) Polyclonal Antibody

Cat. Num. AAA3000525

• Gene Names: FLI1; EWSR2; SIC-1; BDPLT21
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Fli-1; Polyclonal Antibody; Fli-1 (R319) Polyclonal Antibody; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB; FLI1; anti-Fli-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FLI1 (R319) polyclonal antibody detects endogenous levels of FLI1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 419

• Applicable Applications for anti-Fli-1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Fli-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Fli-1 (R319) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Fli-1 antibody

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.

NCBI and Uniprot Product Information

• NCBI GI #: 264681554
• NCBI GeneID: 2313
• NCBI Accession #: NP_001161153.1
• NCBI GenBank Nucleotide #: NP_001161153.1
• UniProt Accession #: Q01543
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: Friend leukemia integration 1 transcription factor isoform 2
• NCBI Official Synonym Full Names: Fli-1 proto-oncogene, ETS transcription factor
• NCBI Official Symbol: FLI1
• NCBI Official Synonym Symbols: EWSR2; SIC-1; BDPLT21
• NCBI Protein Information: Friend leukemia integration 1 transcription factor
• UniProt Protein Name: Friend leukemia integration 1 transcription factor
• UniProt Gene Name: FLI1
• UniProt Entry Name: FLI1_HUMAN

NCBI Description

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11q24.1-q24.3

Cellular Component: nucleus

Molecular Function: DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation