Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit FLCN Polyclonal Antibody | anti-FLCN antibody
FLCN Antibody
Gene Names
FLCN; BHD; FLCL; DENND8B
Reactivity
Human, Mouse, Rat, MonkeyPredicted: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FLCN; Polyclonal Antibody; FLCN Antibody; BHD; BHD skin lesion fibrofolliculoma protein; Birt Hogg Dube syndrome protein; Birt-Hogg-Dube syndrome protein; DKFZp547A118; FLCL; Flcn; FLCN_HUMAN; FLJ45004; FLJ99377; Folliculin; MGC17998; MGC23445; anti-FLCN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Predicted: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
FLCN Antibody detects endogenous levels of FLCN.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
342
Applicable Applications for anti-FLCN antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human FLCN.
Tissue Specificity
Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using FLCN Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using FLCN Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using FLCN Antibody. Lane 1: VERO cells, treated with blocking peptide; Lane 2: VERO cells; Lane 3: Mouse spleen; Lane 4: MCF7.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using FLCN Antibody. Lane 1: VERO cells, treated with blocking peptide; Lane 2: VERO cells; Lane 3: Mouse spleen; Lane 4: MCF7.)
Related Product Information for anti-FLCN antibody
Description: May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Post Translational Modifications: Phosphorylated. Several different phosphorylated forms exist.
Subunit Structure: Interacts (via C-terminus) with FNIP1 and FNIP2 (via C-terminus). This mediates indirect interaction with the PRKAA1, PRKAB1 and PRKAG1 subunits of 5'-AMP-activated protein kinase (PubMed:17028174, PubMed:18403135, PubMed:18663353, PubMed:27353360). Interacts with HSP90AA1 in the presence of FNIP1. Interacts with HSP70, STUB1, CDC37, AHSA1, CCT2, STIP1, PTGES3 and PPP5C (PubMed:27353360).
Similarity: Belongs to the folliculin family.
Post Translational Modifications: Phosphorylated. Several different phosphorylated forms exist.
Subunit Structure: Interacts (via C-terminus) with FNIP1 and FNIP2 (via C-terminus). This mediates indirect interaction with the PRKAA1, PRKAB1 and PRKAG1 subunits of 5'-AMP-activated protein kinase (PubMed:17028174, PubMed:18403135, PubMed:18663353, PubMed:27353360). Interacts with HSP90AA1 in the presence of FNIP1. Interacts with HSP70, STUB1, CDC37, AHSA1, CCT2, STIP1, PTGES3 and PPP5C (PubMed:27353360).
Similarity: Belongs to the folliculin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 45 kDa; 70 kDa
Predicted: 65 kDa
Predicted: 65 kDa
NCBI Official Full Name
folliculin isoform 2
NCBI Official Synonym Full Names
folliculin
NCBI Official Symbol
FLCN
NCBI Official Synonym Symbols
BHD; FLCL; DENND8B
NCBI Protein Information
folliculin
UniProt Protein Name
Folliculin
Protein Family
UniProt Gene Name
FLCN
UniProt Synonym Gene Names
BHD
NCBI Description
This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Research Articles on FLCN
1. FLCN functions as a Rab7A GTPase-activating protein.Negative regulation of EGFR signalling by FLCN.
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Product Notes
The FLCN flcn (Catalog #AAA9611360) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FLCN Antibody reacts with Human, Mouse, Rat, Monkey Predicted: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's FLCN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the FLCN flcn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLCN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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