Rabbit anti-Human, Mouse CDC25C Polyclonal Antibody | anti-CDC25C antibody

Phospho-CDC25C (Ser216) Antibody

Cat. Num. AAA9601029

• Gene Names: CDC25C; CDC25; PPP1R60
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Synonyms: CDC25C; Polyclonal Antibody; Phospho-CDC25C (Ser216) Antibody; CDC 25; Cdc 25C; CDC25; Cell division cycle 25 homolog C; Cell division cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; M-phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; MPIP3_HUMAN; Phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1; regulatory subunit 60; anti-CDC25C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-CDC25C (Ser216) antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216
• Purity/Purification: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 473

• Applicable Applications for anti-CDC25C antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human CDC25C around the phosphorylation site of Serine 216
• Subcellular Location: Nucleus.
• Tissue Specificity: Expressed predominantly in G2 phase.
• Predicted Cross Reactivity: Horse, Dog, Xenopus
• Similarity: Horse (90%), Dog (90%), Xenopus (90%)
• Conjugation: Unconjugated
• Epitope: Phospho Ser216
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CDC25C phosphorylation expression in serum treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9601029 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunohistochemistry (IHC)

(MBS9601029 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CDC25C antibody

Description: A member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
Function: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Subunit Structure: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1.
Post-translational Modifications: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Similarity: Belongs to the MPI phosphatase family.

NCBI and Uniprot Product Information

• NCBI GI #: 567316253
• NCBI GeneID: 995
• NCBI Accession #: NP_001274511.1
• NCBI GenBank Nucleotide #: NM_001287582.1
• UniProt Accession #: P30307; Q96PL3; Q9H168; Q9H2E8; Q9H2E9; Q9H2F1; D3DQB8
• Molecular Weight: Observed: 60 kDa; Predicted: 54 kDa
• NCBI Official Full Name: M-phase inducer phosphatase 3 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25C
• NCBI Official Symbol: CDC25C
• NCBI Official Synonym Symbols: CDC25; PPP1R60
• NCBI Protein Information: M-phase inducer phosphatase 3
• UniProt Protein Name: M-phase inducer phosphatase 3
• UniProt Gene Name: CDC25C

NCBI Description

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]

Uniprot Description

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.